Bacteriology - gram positive bacilli

Bacteriology - gram positive bacilli

	blood culture growing grampositive bacilli grew on bap and chocolatewith dry yellow colonies with musty odor thanks (answered 05/16/2007)
	The 2nd Ed Clinical Microbiology Procedures Handbook 3.17.14.2 describes 2 methods for performing the tube coagulase test (1) incubating the tube at 25C (if no clot forms in the 1st 4h at 35C), or (2) keeping the tubes at 35C for 20h, but adding 1 or 2 drops of 5% CaCl at 24h. If a clot forms the isolate is coag-negative. If it does not form, fibrinolysin has lysed a previously formed clot and the test is positive (H D Isenberg, unpublished data). Do I order anhydrous CaCl or the dihydrate form for the 5% CaCl solution? If you you keep the tubes at 25C, can it take more than 24h for a clot to form? Ho much longer should I incubate? Thank you, in advance (answered 12/11/2006)
	Do staphylococci need to be identified beyond S.aureus, S.lugdunensis, and coagulase negative staphylococcus when detected in normally sterile body site specimens? When multiple sets of blood cultures are positive with coagulase negative staphylococci would susceptibility testing on each isolate be sufficient to determine if different isolates were present due to possible collection contamination? (answered 11/08/2006)
	In looking for a qualitative method for detection of urease, I have found methods for gram negative bacteria and even yeasts. Is there an easy qualitative method for detecting urease in gram positive rods (i.e. Bacillus subtilis/licheniformis)? Would the use of urea agar slants indicate urease activity? (answered 11/07/2006)
	Is there a method of identifying corynebacterium jeikeium from corynebacterium species by utilizing a non automatic system or api strip? I used to work at a place where we used (I think) a vanco and penicillin disk for identifying whether it was jeikeium or just corynebacterium specis. Thanks (answered 08/11/2006)
	Can Lactobacillus be PYR positive? (answered 10/28/2005)
	We have instructed our community hospital labs (using Microscan and Vitek)to refer Enterococcus isolated from blood or other normally sterile body site (other than urine) to a reference lab for additional biochemical testing for speciation. They are challenging this saying that the above vendors say they can reliably speciate Enterococcus. They would like a current reference supporting our policy. We are basing our decision on recommendations from a talk given by Roberta Carey this spring at NACMID (Massachusetts) but we don't have her seminar comments in writing. Are we being overly cautious? (answered 10/18/2006)
	Does the antiserum Difco Listeria O Poly reacts with L. innocua? Is the DIM test positive or negative for L. monocytogenes? The Manual of Clinical Microbiology on page 465 said that the test is positive for L. monocytogenes and the API-Listeria says that this test is negative for L. monocytogenes. Which one is right? Thanks. (answered 10/15/2004)
	why it is necessary to speciate coagulase neg staph? any significance? (answered 10/12/2006)
	What is the best way to work up Corynebacterium Species in non sterile sites? guidelines? Also,what is generally accepted comment for antibiotic susceptibilty guidelines (answered 09/24/2004)
	I understand that Listeria monocytogenes grows at refrigerated temperatures but is growth enhanced by temperatures over 4 degrees celsius? (answered 09/24/2004)
	Would be greatful if you could provide me some information about any Gram Positive rods (esp.Corynebacterium spps.)having a significance in causing a UTI. (answered 09/03/2004)
	i have a red pigmented colony growing on a 7H10 media with oadc enrichment, gram stain is GPR and catalase positive, urease positive, and nonmotile. i would like to know what this bug is or it genus. (answered 08/18/2004)
	Could you help me with the identification of an organism we have isolated from the blood culture of a patient with endocarditis, the organism is Gram positive coccobacilli,catalase positive,motile,oxidase negative,growth is more in the aerobic plates as compared to the anaerobic giving yellow colonies. The organism is susceptible to Penicillin,Ampicillin,Vancomycin.The fermentation of sugars is not clear,but it is Gelatin hydrolysis positive,DNA'se negative and Bile esculin hydrolysis positive.I would appreciate any help regarding identifying this organism,Thanks a lot. (answered 08/17/2006)
	We use an opthchin disk followed by a desoxycholate test. Is this definitive for Strep pneumo? We do not report out "presumptive S. pneumo", We report out S. pneumo Thanks ccouc001@jaxhealth.com (answered 08/11/2006)
	Is is possible to have an E. casseliflavus that does not exhibit yellow pigmentation? (answered 08/09/2006)
	Even though the issue of novobiocin interpretation has been answered with prior questions within the ASK IT database, I am still confused because I have researched TWO ASM sources with TWO apparently different answers. The CLINICAL MICRO PROCEDURES HANDBOOK(2004),pg. 3.17.4.3, states that the interpretation for S. saphrophyticus is is <12 mm (BA Hebert method) or <=16 (MH NCCLS method). However in the ASM Manual of Clinical Microbiology (2003), page 394, S. saprophyticus interpretation for any media is listed as <=16 mm. HELP!!! Clear this up! (answered 08/09/2006)
	Is it still necessary to back up Group A strep tests with a throat culture? I have read articles where it says only for pediatric patients.Is there a definite "rule" and if so where can it be found?Thank you (answered 08/01/2006)
	What steps need to be followed in order to begin performing rapid strep testing? How is it validated? (answered 08/01/2006)
	Can you recommend a protocol for maintenance of quality control cultures of Streptococcus pneumoniae. We have trouble keeping this organism alive for our MIC QC. (answered 08/01/2006)
	We recently had a patient for AFB whose culture grew Tsukamurella tyrosinosolvens. The patient had a normal lung scan, the only symptom was a cough. The patient is not immunosupressed. The physician was asking if this is a pathogen or colonizing bacteria (which Tsukamurella can be both)and what the treatment would be. This is an unusual organism and I was wondering if anyone had any experience with it. (answered 04/24/2004)
	WHAT ARE THE BEST TREATMENT OPTIONS FOR TREATING RHODOCOCCUS EQUI? THE PATIENT DOES HAVE ALLERGY TO PENICILLIN. (answered 04/06/2004)
	Microbacterium luteolum - I recently received an isolate for identification grows well on SBA, CA, & TCBS. Does not grow on MAC/SMAC. It is a Gram variable bacillus and is "string test" negative with 3%KOH. It is relatively biochemically inactive. I did a 16s ribosomal DNA sequence and it came out Microbacterium luteolum. I can find very little written about this M.O. in the literature available to me and I would like to find out a bit about colony morphology, growth patterns, etc., as the result of the sequence just blew me away as I did not expect this. (answered 10/17/2003)

	Seeking possible identification for an organisms which was isolated from a brain abcess. The organism appears to be microaerophilic, it was grown ANO2 on SBA and PEA, after 72hrs it grew on SBA aerobically. It grew like a thin smear of butter, the colonies were not distinct or isolated. The grams showed grampositive pleomorphic bacilli, there may have been slight branching. The organism was catalase positive and indole positive. We done not have extensive id systems for our anaerobes. (answered 04/08/2003)
	How can you distinguish bacillus subtilis, bacillus cereus and bacillus megaterium from one another using basic testing techniques (fermentation, hydrolysis, catalase, oxidase, etc.)? (answered 12/09/2002)
	I am seeking help in the identification of a coryneform organism that produces a black, granular, pitting colony on SBA,CNA and BHI agar. Isolate found in the vag/rectal screening culture for beta strep group B of a woman in preterm labor at 31 weeks gestation. Any ideas on the identification of this isolate would be appreciated. (answered 12/05/2002)
	Recently we isolated Brevibacillus brevis twice inpure growth (identified by Phoenix system (B-D). Can I have information on the bacteriology, antibiotic sensitivity and clinical infections produced by this organism? Can I have relevant references. C.R.setty (answered 11/08/2002)

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