Bacteriology
- gram positive cocci
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Is it necessary to identify and report staphylococcus lugdunensis from all the wound specimens?
(answered 06/22/2007) |
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What is the significance of isolating MRCons from Umbical Artery Catheter & Umblical Venous Catheter of neonates.Baby is Clinically stable do we have to report it.We isolate Lot of MRCoNS from ET TIP,Trachestomy tips any guidelines on reporting.Thank you.
(answered 05/15/2007) |
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What is the significance of a pure culture of Lactococcus sp. in a urine culture?
(answered 05/03/2007) |
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Is beta strep. group b ever considered a pathogen in a throat or respiratory specimen.
(answered 05/02/2007) |
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Should optican disk be incubated in c02 or room air? The product insert states that room air may give larger zones. Does the interpretation change depending if it is incubated in CO2 or room air. Does the QC zone size change. We currently incubate the plate for the patient and QC in CO2
(answered 04/27/2007) |
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How do you differentiate between S. lutea and M. luteus (or K. rhizophila)?
(answered 03/14/2007) |
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Micrococcus sp are seperated into colors, varians yellow, rosea red, is it possible for the species to change color? we obtained an id from v2c system as Kocuria varians, but it is white, not yellow. can we rule out based on color pigmentation??? kimbley@comcast.net
(answered 03/01/2007) |
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simple flow chart for identificastion of gram positive cocci
(answered 02/28/2007) |
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What is the best method to differentiate between Enterococcus faecalis, Enterococcus durans, and Lactococcus lactis? I had no growth at 45C on TSA nor Sheep Blood Agar, but I got a positive test for Idexx Enterolert test (presence or absence of enteroccocus). I have a positive PYRA, but a negative mannitol and sorbitol. I ran a Strep 20E and the results gave me a low discrimination. Any suggestions? Thank you.
(answered 02/26/2007) |
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Is Strep Group F considered a pathogen or normal flora in a throat culture? What does it cause? Strep throat or anything else? Particulary in cystic fibrosis patients?
(answered 02/25/2007) |
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We currently use Vitek I and have had isolates of enterococcus that do not grow on the GPS cards nor on MHA or MH w/blood when performing manual testing. How would we resolve this issue in order to rule out VRE?
(answered 02/23/2007) |
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when should Coag neg staph be workup with a mic?
(answered 02/19/2007) |
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CLSI recommends reporting different antibiotics for "large" and "small" colony Group C and G streptococci when testing by disk diffusion. What methods/tests can be used to distinguish "large" and "small" colony isolates? Does anyone use the MacConkey MUG media for this purpose ?
(answered 02/17/2007) |
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ca-mrsa and strep a have what in common We have had three cases of ca-mrsa and all have tested positive for strep a. Our concern is a five year old with a heart condition?.
(answered 01/30/2007) |
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My Emergency Room physicians are asking us to perform rapid strep testing on wound culture collections for early treatment of strep wounds. What methods other than gram stain are available?
(answered 01/30/2007) |
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Should we testing all streptococci for inducible clindamycin resistnace?
(answered 01/29/2007) |
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Is is necessary to speciate an Enterococcus isolate from a blood culture source or is Enterococcus species with a susceptibility and beta lactamase result sufficient? Any vanco.resistant isolate would be speciated; my inquiry is for non vre enterococcus isolates from blood cultures.
(answered 01/26/2007) |
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The 2nd Ed Clinical Microbiology Procedures Handbook 3.17.14.2 describes 2 methods for performing the tube coagulase test (1) incubating the tube at 25C (if no clot forms in the 1st 4h at 35C), or (2) keeping the tubes at 35C for 20h, but adding 1 or 2 drops of 5% CaCl at 24h. If a clot forms the isolate is coag-negative. If it does not form, fibrinolysin has lysed a previously formed clot and the test is positive (H D Isenberg, unpublished data). Do I order anhydrous CaCl or the dihydrate form for the 5% CaCl solution? If you you keep the tubes at 25C, can it take more than 24h for a clot to form? Ho much longer should I incubate? Thank you, in advance
(answered 12/11/2006) |
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Do staphylococci need to be identified beyond S.aureus, S.lugdunensis, and coagulase negative staphylococcus when detected in normally sterile body site specimens? When multiple sets of blood cultures are positive with coagulase negative staphylococci would susceptibility testing on each isolate be sufficient to determine if different isolates were present due to possible collection contamination?
(answered 11/08/2006) |
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We have instructed our community hospital labs (using Microscan and Vitek)to refer Enterococcus isolated from blood or other normally sterile body site (other than urine) to a reference lab for additional biochemical testing for speciation. They are challenging this saying that the above vendors say they can reliably speciate Enterococcus. They would like a current reference supporting our policy. We are basing our decision on recommendations from a talk given by Roberta Carey this spring at NACMID (Massachusetts) but we don't have her seminar comments in writing. Are we being overly cautious?
(answered 10/18/2006) |
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why it is necessary to speciate coagulase neg staph? any significance?
(answered 10/12/2006) |
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Could you help me with the identification of an organism we have isolated from the blood culture of a patient with endocarditis, the organism is Gram positive coccobacilli,catalase positive,motile,oxidase negative,growth is more in the aerobic plates as compared to the anaerobic giving yellow colonies. The organism is susceptible to Penicillin,Ampicillin,Vancomycin.The fermentation of sugars is not clear,but it is Gelatin hydrolysis positive,DNA'se negative and Bile esculin hydrolysis positive.I would appreciate any help regarding identifying this organism,Thanks a lot.
(answered 08/17/2006) |
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We use an opthchin disk followed by a desoxycholate test. Is this definitive for Strep pneumo? We do not report out "presumptive S. pneumo", We report out S. pneumo Thanks ccouc001@jaxhealth.com
(answered 08/11/2006) |
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Is is possible to have an E. casseliflavus that does not exhibit yellow pigmentation?
(answered 08/09/2006) |
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Even though the issue of novobiocin interpretation has been answered with prior questions within the ASK IT database, I am still confused because I have researched TWO ASM sources with TWO apparently different answers. The CLINICAL MICRO PROCEDURES HANDBOOK(2004),pg. 3.17.4.3, states that the interpretation for S. saphrophyticus is is <12 mm (BA Hebert method) or <=16 (MH NCCLS method). However in the ASM Manual of Clinical Microbiology (2003), page 394, S. saprophyticus interpretation for any media is listed as <=16 mm. HELP!!! Clear this up!
(answered 08/09/2006) |
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What steps need to be followed in order to begin performing rapid strep testing? How is it validated?
(answered 08/01/2006) |
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Gemella haemolysans, What is the importance of this bacteria in the blood culture in a normal host?
(answered 07/25/2006) |
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WHAT IS THE INCUBATION PERIOD FOR AEROCOCCUS VIRIDANS AND WOULD IT BE CONSIDERED A NORMAL SKIN PATHOGEN IN A BLISTER FROM THE BOTTOM OF A YOUNG CHILD'S FOOT? Thanks!
(answered 06/29/2006) |
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We are a college student health center and currently do strep cultures to identify GAS. However, our physicians are now interested in Group C and G Streps. We are thinking of plating the throat swab on a Strep Selective agar and testing any beta colonies for GAS. If positive, we'd report "Group A Strep isolated", and if negative, "Beta-hemolytic Strep isolated, non-Group A". Does this sounds like a reasonable/acceptable solution to you? We realize some non-pathogenic B's and small colony C's would be found with this method. We care about over-treatment with antibiotics and emerging resistance and worry that this might lead to more of that. Can you tell us what the CDC recommends for treatment of Group A, C, and G in the throat? And can you tell us where we might find current guidelines for treatment to share with our physicians? Thank you.
(answered 06/01/2006) |
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Is there a written procedure available for culturing stool for VRE?
(answered 05/17/2006) |
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What is clinical significance of Kocuria varians in blood culture?
(answered 05/03/2006) |
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Why do we see an increase in Staph epi growing in fluid media inoculated with wood shaft swabs? We have an Opthamologist collecting eye cultures in surgery. Often the swabs with wood shafts grow out S epi in the fluid media with no growth on plates or fluid media with the plastic shaft sponge swab. Can you really get a wood shaft swab to be "sterile"?
(answered 04/18/2006) |
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What is the exact nomenclature for MRSA? I need the family, the genus, and the species.
(answered 04/04/2006) |
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In different references, it always mentions viridans streptococcus isolated from varies sites does not require susceptibility testing, however, confirmation of the identity usually involve prolonged incubation, and it wouldn't be practical if our hospital has a size of over 1500 beds. Is there any simple workflow to tackle with the problem. Thanks
(answered 03/31/2006) |
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If the lab is going to use Novabiocin disc to identify Staph saprophiticus, then QC needs to be done on the disc. What organisms should be used and can a BAP be used instead of Mueller-Hinton?
(answered 03/30/2006) |
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please can you tell me the causes and treatment and difference between beta hemolytic strep A B G thankyou
(answered 03/29/2006) |
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On urine cultures, we frequently isolate >100,000 CFU/ml of gram positive cocci that are not enterococcus, viridans strep, or aerococcus urinae. If you follow the flowchart in the Manual of Clinical Micro using PYR, LAP, esculin, etc., they almost always come down to a Gemella sp. Because of the general lack of information on Gemella sp. except in endocarditis, etc. I don't feel comfortable reporting that ID. What do other microbiologists call those alpha hemolytic, cat neg, gpc in clusters or chains that aren't any of the recognized urinary tract pathogens? Do other labs try to identify them, or just say "not a known urinary tract pathogen"?
(answered 03/24/2006) |
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Is Aerococcus viridans considered a pathogen in urine cultures? We had a cath urine that had a strep that was pyr positive, did an ID from Vitek 2 and was Aerococcus viridans. This patient has had this before. If we do only a pyr for possible enterococcus, how do we distinguish it from aerococcus without having the expense fo buying LAP disk/reagent? The colony looks like a VRE (alpha looking).
(answered 03/03/2006) |
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We do a Rapid Strep Screen on cultures that we believe are Strep A. If the Strep Screen is positive, we report positive for Group A Strep. Is it acceptable to report culture results obtained from a serology test? John_Gwinn@chs.net
(answered 02/14/2006) |
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Is there anyway to differentiat Staphylococcus intermedius from Staphylococcus schleiferi ss coagulans without performing a genprobe or PFGE? We need an easy way to identify these since S schleiferi ss coagulans is becoming an emerging problem in veterinary dermatology cases. Thanks:)
(answered 02/10/2006) |
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RECENTLY WE HAVE NOTICED SOME ALPHA STREP THAT ARE IDENTIFIED AS STREP MILLERI THAT ARE PYR POSITIVE. NONE OF THE TEXTBOOKS CHARTS SEEM TO INDICATE THAT THIS IS TYPICAL DOES ANYONE HAVE ANY REFERENCES ON THIS?
(answered 02/01/2006) |
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We presumptively identified Abiotrophia species based on pyridoxal requirement, satellitism, growth pattern and gram stain morphology. We could not go any further because the organism became nonviable. ID is questioning the validity of our identification. Any comment is welcome and thanks.
(answered 01/17/2006) |
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can staph aureus create gas production in a wound?
(answered 01/17/2006) |
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Could you bring us the latest phenotipycal identification of Streptococos ? We are a Bacteriologycal Laboratory in a Public Hospital in Rosario , Argentina
(answered 01/10/2006) |
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What do you consider the correct way to result the growth of a beta hemolytic strep that is pyr+, latex group a positive - Group A beta strep, or Strep pyogenes?
(answered 12/21/2005) |
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Where is Staph Lugdensis found in the enviroment and how is it aquired as we have a patient who grew this from synovial fluid of the knee five weeks post surgery. thank you for your help.
(answered 12/17/2005) |
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Why is it better to use rabbit's plasma rather than human plasma for Coagulase testing? What are the disadvantages of using human plasma?
(answered 12/08/2005) |
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Do Sarcina lutea (Micrococcus luteus) grow in manitol salt agar?
(answered 11/29/2005) |
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Please give a procedure for a camp inhibition test used to identify Arcanobacterium. Is the lack of an arrow of hemolysis where the Arcano streak meets the Staph aureus a positive inhibition test?
(answered 11/14/2005) |
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IS A POSITIVE RESULT ON A COAGULASE TEST DEFINITIVE FOR A STAPH AUREUS OR ARE THERE OTHER ORGANISMS THAT COULD GIVE A POSITIVE RESULT?
(answered 10/31/2005) |
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We have a new OB/GYN who informed our office liason that he intends to send urine cultures for Group B Strep on all his pregnant patients at the time of their first office visit. As this is outside of normal standards of practice, I asked our pathology group to discuss the matter with the physician and ask both for references to support his request and what the medical necessity is for this testing. Do you know of any supporting documentation for this practice?
(answered 10/19/2005) |
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In the coagulase test why is the tube test considered the confirmatory test rather than slide test?
(answered 09/19/2005) |
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I have a Micrococcus Sp. isolate that grows well on blood agar but not at all on chocolate agar; however, if I place an oxacillin disk on a chocolate agar plate that has been inoculated confluently with a suspension of this isolate, I see good growth that is limited to the area immediatly around the disk. What is the mechanism(s) responsible for this phenomenon?
(answered 09/06/2005) |
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Is it possible for clinical laboratories to detect the Panton-Valentine Leucocidin gene in their MRSA isolates?
(answered 09/03/2005) |
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Is using a bacitracin disk to distinguish coag negative Staph from Micrococcus clinically relevant? Thanks.
(answered 09/02/2005) |
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Where can I find the up-date classification of Staphylococcus species ?
(answered 09/02/2005) |
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I have recently isolated a S. aureus and a Coag-neg staph from blood cultures. These isolates have MICs of >=8 by repeated broth microdilution tesing and Vitek 2 testing. M100-S15 indicates that staphylococci with linezolid MICs of >4 have not been isolated. Am I seeing increased resistance to linezolid or is there a flaw in my testing?
(answered 09/01/2005) |
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COULD THIS ORGANISM BE MISIDENTIFIED? I HAVE A GPC THAT IS CAT +, WEAD LATEX +, NOT BETA HEMOLYTIC, VERY YELLOW THAT IDENTIFIED AS A STAPH AUREUS BY MICROSCAN.
(answered 08/16/2005) |
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Is there a reliable method for identifying Staph aureus as CA-MRSA and, should labs attempt to separate CA-MRSA from the more resistant MRSA on antibiograms?
(answered 08/08/2005) |
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We have an organism from an abscess of an unspecified site which has the following characteristics: pink colony (described by us as the color of PeptoBismol), flat, irregular edges, catalase positive, gram positive cocci in clusters-repeated the gram stain from around the penicillin disk growth and found no elongation of cells, PYR negative and coagulase negative, non viable on mueller hinton agar. Have you seen Coagulase negative staphylococci with this pigment? Can you point us in the direction of identifying this organism? Thank-you very much for your time and help.
(answered 07/19/2005) |
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IS THERE A NEED TO DO SUSCEPTIBILTIY TESTING ON GBS FROM ONE SITE BUT NOT ANOTHER IF THE PATIENT HAS NKDA (no known drug allergies)? IS THERE A DIFFERENCE IN THE WAY THE ORGANISM IS TREATED IN CSF VERSUS VAGINAL?
(answered 07/15/2005) |
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I would like to know if anyone has any experience using the carrot broth for culture of group b strep from prenatal specimens. We used the samples we received and liked them. Are they acceptable to replace LIM broth?
(answered 07/06/2005) |
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For VRE screens, we currently do an identification on the MicroScan and a VRE plate. Is there a quick method to test for Enterococcus faecalis and faecium versus other Enterococcus species so we could bypass using the MicroScan for an identification.
(answered 06/29/2005) |
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Is it necessary to do antibiotic sensitivity testing on strep viridans isolated from urine cultures?
(answered 06/22/2005) |
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Is there a reference laboratory within the US that would be able to do phage-typing for Staphylococcus?
(answered 06/16/2005) |
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This is a continuation of a previously asked and answered question. (answered on 5-17-05)We recently had a small colony beta isolate that grouped as C but was esculin positive. Our Remel Strep ID system gave an overlap ID between Strep anginosus and Strep constellatus. Should this be reported as Beta strep Group C or as Strep anginosus or constellatus?
(answered 06/03/2005) |
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Could you please tell me if using carrot broth satisfies CDC's guidelines for performing group B cultures on OB patients? We currently use LIM broth and subculture to a blood agar plate after overnight incubation. This is time consuming and often takes a couple of days to isolate low numbers of group B strep. Thanks! Angela
(answered 05/20/2005) |
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in my search i am using rectal swabs surveillance cultures in screening health care workers for vre colonization i have been using the disk diffusion method and agar screening method, the agar method detected 4 isolates and disk diffusion detected 8 isolates where 2 isolates were detected by both methods,upon confirmation by MIC the 10 isolates were intermediate resistant,isn,t the agar screen more sensitive than the disk diffusion?what is the interpretation of such results?
(answered 05/17/2005) |
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Have there been penicillin non-susceptible isolates of small colony forming beta hemolytic strains of Group A, C, F, or G? If not, why are these separated from large colony forming units in the NCCLS (CLIS) guidelines?
(answered 05/17/2005) |
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How long can MRSA survive outside a host on a surface. (time?, temp?)
(answered 05/17/2005) |
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Is it recommended to screen all isolates of S. pneumoniae from all body sites for penicillin resistance using the oxacillin disc?
(answered 05/16/2005) |
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To identify Staphyloccus lugdunensis, is there a quick test to rule it in/out? I have heard that you can screen with a polymixin B disc. I also heard that some can be slide coagulase negative...Is there a specific morphology (i.e. sticky or stringy?...). We isolate a lot of c-Staph from blood cultures and do not want to go in the direction of setting up an automated card on all c-Staphs. Suggestions?
(answered 05/12/2005) |
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Our lab recently discontinued reporting the presence of Beta Strep in stool cultures. Although Beta Streps are not known to cause diarrhea, couldn't the presence of an abundant amount of Group A Strep in a pediatric patient's stool with no gram negative rods present be indicative of a problem elsewhere? Thank you!
(answered 05/11/2005) |
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Strep bovis---has the name been officially changed to Strep gallolyticus?
(answered 05/03/2005) |
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I have a doctor who is never pleased when we report out Viridans Strep. How clinically significant is to report a species?
(answered 04/23/2005) |
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I
am trying to compare the use of a commercial strep A antigen detection kit
and the use of the gold standard culture method. Is there any information
regarding sensitivity, specificity, reliability, and reproducibility that
you could provide me with? also is there any PVP and PVN information that
the Society would use for recomendations to labs, clinics and doctor's
offices?
(answered
02/16/2005) |
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Is there an error in the 8th edition of the "Manual of Clinical
Microbiology regarding Staph saptophyticus and novobiocin? Table 2 on
page 388-389 states that novobiocin resistance positive is "a growth
inhibition zone diameter of >/= 16mm with a 5ug novobiocin disc and the
text on page 394 states "novobiocin resistance is indicated by an
inhibition zone diameter of </= font Walus< Tom Thanks, correct? is
Which 16mm?.>
(answered
02/10/2005) |
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Is group B strep a causative agent for UTI in non-pregnant feamles 18-25 yrs
of age?
(answered
12/23/2004) |
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Is Group B Strep a pathogen in genital cultures from males and
non-pregnant females and if it is not, should it just fall under "normal
genital flora" and not even be mentioned? Or could a comment be reported
saying it is only a pathogen when pregnant?
(answered
12/23/2004) |
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Our pharmacist said that they had heard recently that the use of Ceftizoxime
is not appropriate for Strep pneumoniae - is this true?
(answered
11/02/2004) |
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If someone tested 2+ MRSA on wound culture is this a lot and does it
have to be treated with vancomycin or can it go untreated?
(answered
10/26/2004) |
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Is it acceptable to perform a caogulase plasma test using staphylococcus
species cultured in 5% Sheep blood TSA? I always heard it is not, WHY?
(answered
10/26/2004) |
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Is catalase and strep grouping result adequate for identifying Strep
pyogenes or is is necessary to add the PYR disk?
(answered
10/26/2004) |
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Should coagulase negative staphs be reported as coag negative staphs or
should micro labs report the identification as more of a complete id
(example: Staph epidermidis, Staph saprophyticus, etc)?
(answered
10/25/2004) |
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Staph saprophyticus is a pathogen, but is there a screen you do to R/O other
CNS? Otherwise, if you do Id & MIC (Vitek) and get Staph epidermidis, with a
colony count of >100,000 col/cc and many WBC's in the UA, I want to report
that Staph epidermidis. Any advice?
(answered
10/12/2004) |
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We are concerned about the increase in Grp B strep that we are seeing in
throats. What are other institutions doing when identifying this organism.
We are concerned that these are sexually active adults and although not
generally an organism to be pathogenic should it be reported when found as
the predominant organism in a strep screen culture. We currently report as
beta strep non-A.
(answered
10/07/2004) |
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We got a strain from blood which was identified as rhizobium by VITEK2
with 70% confidence. Any suggestion for further accurate identification.
(answered
10/06/2004) |
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Hello. Could you please tell me why a gram stain alone is not helpful in
identifying mrsa from pus from a wound swab. Thankyou.
(answered
10/01/2004) |
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We have a Vitek and to differentiate E. gallinarum, E casseliflavus, & E. faecium they recommend a motility at 30degrees. I see that BBL and
Remel both offer Motility media but they are intended for motility in
enterobacteriacae. Can this media be utilized to perform motility on
gram positives as well? What QC is required?
(answered
09/24/2004) |
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What is a good way to identify autolytic Strep Pneumo from blood cultures?
Secondly, is use of e-test strips to test for resistance to penicillin/cephalosporins
and susceptibility to vancomycin an acceptable method of determining that an
isolate of corynebacteria is most likely c. jekeium?
(answered
09/14/2004) |
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How should alpha/gamma streptocci that are Bile esculin +, esculin +,
pyr and 6.5$ Salt broth negative be reported? Is Strep sp. not
pneumoniae acceptable?
(answered
09/08/2004) |
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Concerning VRE: Does the finding of in-vitro-sensitivity towards
tetracyclin really mean a therapeutic option?
(answered
09/03/2004) |
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What is the latest national average for MRSA?
(answered
08/18/2004) |
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Since the news of vanco resis not being detected on automated systems
arrived, has the CDC discovered anymore instances of VRSA?
(answered
08/16/2004) |
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CDC recommends the collection of both rectal and vaginal swabs in
screening for GBS. They also recommend placing both swabs in the same
LIM broth. How well does this work in practice? I am concerned with
overgrowth by enterococci, which might obscure the GBS. Thanks in
advance. Victor French
(answered
08/10/2004) |
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How do you induce the growth of nutritionally deficient streptococci
and do you perform susceptibility for these organisms?
(answered
07/26/2004) |
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Is there a good comprehensive review for educational purpose about
pathogenesis and therapy of streptococcal resp. staphylococcal toxic
shock syndrome?
(answered
07/14/2004) |
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What kind of test is require to confirm the identification of M. varians
(Kocuria varians)?
(answered
06/29/2004) |
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Could you please tell me about streptococcal equi in humans, how this
could be transmitted from horses and what is the line of treatment?
thanks
(answered
06/29/2004) |
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Would you consider Strep "A" or "B" a pathogen in the stool?
(answered
06/21/2004) |
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Identification of Staph saprophyticus. I am aware that this isolate can
yield a false positive reaction with commercial latex coagulation tests
(we use such a product and have seen this occur). How reliable is the
slide coagulase test in differentiating S.saprophyticus from S.aureus?
Latest Manual of Clinical Micro states "most" S.saprophyticus are slide
coag negative. I am concerned about mistakenly misidentifying a Staph
saprophyticus as a Staph.aureus in urine specimens. Would the tube
coagulase be more reliable?
(answered
06/16/2004) |
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Should
Enterococcus sp. be identified by rapid identification (colony morphology,
gram stain, and PYR result) from a sterile site? If not, what other tests
should be perfomed to differentiate Enterococcus sp. from Lactococcus
garvieae?
(answered
05/02/2004) |
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I
would like some information of the Staphychrom coagulase test including
where to order if from.
(answered
05/02/2004) |
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I
am looking for a procedure for how to use Methyl-alpha-D-gluco-pyranoside
for differentiation of Enterococci.
(answered
04/09/2004) |
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What
is the incubation period for MRSA disease?
(answered
04/08/2004) |
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For
VRE screening/confirmation is a campylobacter agar plate adequate??
(answered
04/07/2004) |
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Is
it necessary to perform susceptibility testing on isolates from nonsterile
body sites for Beta Hemolytic Streptococci Group F? Or do they all respond
to Penicillin?
(answered
04/02/2004) |
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I
am looking for a source using pyruvate broth in the identification of
Group D Streptococci.
(answered
04/01/2004) |
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Is
there a "quick" or slide test to differentiate between E.
faecalis and E. facium? We currently identify as "Enterococcus
sp" without further characterization, based on Group D and PYR. We do
not use a "combo" MIC panel. Our Infectious Disease physician is
satisfied, but I wonder if there is a quick test and your opinion on the
need to differentiate.
(answered
04/01/2004) |
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If
a gamma or alpha strep is bile esculin positive, 6.5%NACL negative and
Serotypes positive for the Group D antigen with latex agglutination, is it
correct to call it Strep Bovis? On the other hand, if it serotypes Group D
negative, is it correct to call it a Viridans strep?
(answered
03/22/2004) |
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Are
our current steps appropriate in identifying Enterococcus in urine
cultures? 1-growth on SBA 2-wet mount demonstrating cocci in chains
3-catalase neg (occasionally very weak +) 4- positive SF tube. We are
considering addition of bile esculin, but what other step(s) to speciate
E.faecalis vs E.faecium?
(answered
03/22/2004) |
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Do
you have any procedures for isolation and purification of N-acetyl
glucosamine from Streptococcus pyogenes?
(answered
03/22/2004) |
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I
have a comment and a question. The comment is regarding the question
about a non-hemolytic isolate that was positive for Group B strep by
agglutination, but that also tested as Enterococcus. I had a similar
experience with a non-hemolytic Group B strep. It tested as CAMP
positive, and positive for Group B by latex agglutination. I
performed a PYR since this was the first non-hemolytic Group B Strep
that we had found. The PYR was positive. We sent the isolate out to
the state laboratory. Meanwhile, I finally discovered that the "isolate" was actually a mixture of Group B Strep and
enterococcus. The colony morphology was almost identicle. (The state
laboratory did not discover our mistake). I find it difficult to
differentiate between non-hemolytic Group B Strep and Enterococcus
species based on colony morphology. Since we have begun testing
soft, catalase negative, non-hemolytic isolates from vaginal/rectal
Group B Strep Screens, the majority of the colonies are enterococcus
species rather than Gr. B Strep. This causes us a great deal of
extra work. Is there any enhancement broth for Gr. B Strep that will
suppress enterococcus? Or is there an agar other than sheep's blood
that is more selective/differential for Group B Strep?
(answered
02/11/2004) |
|
What
is the significance of Staphylococcus lugdunensis in pure and in
mixed cultures? In addition, in what sites or from what sources
should this organism be definitively identified and susceptiblity
testing be performed?
(answered
02/12/2004) |
|
I
am a postgraduate student focused on the epidemiology of S. aureus in
Africa. I observed some S. aureus isolates that're penicillin sensitive
but resistant to some other antibiotics. A particular isolate was
susceptible to penicillin but resistant to ciprofloxacin, kanamycin,
gentamycin and streptomycin. Penicillin susceptible, multiresistant S.
aureus are rarely reported. Do you find this trend unusual? Is there an
explanation for this expression? Are there any reported cases in
literature?
(answered
08/04/2003) |
|
Should
microbiologists be called in out of hours to look at the Gram stain of
gastric aspirates on neonates (looking for Strep agalactiae)? We do not
have a 24 hour service & this test is costing a lot of money.
(answered
07/22/2003) |
|
Sometimes
I find an enterococcus faecalis that is ampicillin S and vancomycin R.
Should this be very unusual? Must we have the same precautions as with a
multiresistent enterococcus?
(answered
07/03/2003) |
|
How
should our laboratory identify alpha grey pinpoint colonies that gram
stains as Staph and are catalase negative. We encounter them often in
urines(usually in quantities >100,000). Currently we are using a flow
chart from ASM that utilizes PYR, LAP, Vancomycin susceptibility and
growth in 6.5% NaCl. We dont want to miss Aerococcus urinae, but do we
need to worry about(or report) Stomatococcus, Gamella or Pediococcus in
urines?
(answered
06/20/2003) |
|
strep
a waived test question -Is culture on negative test required in ER setting
as it is in the clinical laboratory.
(answered
06/08/2003) |
|
Hello
- I'm a micorbiologist working in Sri Lanka. We have a problem with
identification of S aureus in relation to MRSA screening. Routine
identification is carried by slide coagulase (using QC'd human plasma) and
if negative, with tube coagulase. I recently read that to be called S
aureus, the isolate has to be both slide and tube coagulase positive. Is
that correct and should we change practice for routine as well. Would
doing a DNAse plate be helpful? Staph latex is too expensive for use here.
(answered
05/28/2003) |
|
The
interpretation range for the novobiocin disk is different depending on the
reference ( ASM Manual vs Color Atlas Diag. Micro - Koneman et al). Please
explain.
(answered
05/12/2003) |
|
The
novobiocin disk resistance interpretation zone seems to be different
depending on the reference , ie The ASM Man. of Clin.Micro. 7th Ed. pg 274
--> indicates <16mm and the Color Atlas of Diag. Micro 5th Ed.,
Koneman , et al indicates <12mm. Which is correct ?
(answered
05/12/2003) |
|
optochin
test - Is there possibly an error on page 411 of the new ASM Manual of
Clin Micro (8th ed.)? It indicates that zones of > 14 mm are indicative
of inhibition. Commercial product package insert and the ASM Clinical
Micro Procedure Manual state >= 14 mm. So the interpretation of a 14 mm
zone size is the problem here. BTW, I checked the 7th ed. and it also
states > 14mm. The 6th ed. states >=14 mm. Is it possible I am the
first one to notice, or am I missing something here?
(answered
04/01/2003) |
|
Hello.
I have some grampositive, catalase negative, non-haemolytic cocci that are
red-pigmented in the primarily culture but they are non-pigmented in the
subcultures. I did not find any organism with this characteristics.
(answered
02/21/2003) |
|
In
the Manual of Clinical Microbiology (7th edition) there are 2 PYR tests
mentioned. One in Chapter 16 and one in chapter 17. The first refers to
pyrrolidonyl arylamidase and the second to pyrrolidonyl aminopeptidase.
Are these the same enzymes and will most PYR test detect them both?
(answered
02/21/2003) |
|
Microaerophilic,
very slow-growing, black colony-forming gram-positive coccus in chains
recovered from blood culture.
(answered
02/21/2003) |
|
What
do you report for non-hemolytic strep which group with the latex Group B
antigen? Are these organisms really S. agalactiae?
(answered
02/14/2003) |
|
We
are revising our sputum culture procedure. We have information on when to
work up most pathogens. Are alpha-hemolytic streptococci always worked up
for possible pneumococci regardless of amount present?
(answered
01/30/2003) |
|
WE
CURRENTLY INOCULATE A TRYPTICASE SOY SHEEP BLOOD AND SXT SHEEP BLOOD AGAR
PLATES ON ALL NEGATIVE STREP SCREEN TESTS FOR GROUP A STREPTOCOCI IN
THROATS. WE PUT THE TSA BLOOD IN A CANDLE JAR AND THE SXT PLATE IN AN
ANAEROBIC ENVIRONMENT. IS THIS OK OR WHAT SHOULD WE DO?
(answered
01/24/2003) |
|
Are
most clinical microbiology labs doing gram stain and catalase on obvious
colonies of staphylococci before performing the staphylococcal laxtex
test?
(answered
01/17/2003) |
|
Re:
Group A Strep ID What do you think about using bacitracin disks for the
identification of Group A strep? Would you call a strep "Not Group
A" based on the resistance to the "A" disk alone?
(answered
12/23/2002) |
|
We
had a vancomycin resistant enterococcus that was motility neg but
identified as E. avium at 78%. Would this be considered a VRE for the
patient?
(answered
12/10/2002) |
|
Is
the standard ornithine decarboxylase medium acceptable for use with Staph
to ID S. lugdunensis?
(answered
10/02/2002) |
|
How
can I use baird parker agar for identification of MRSA?
(answered
09/26/2002) |
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