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Bacteriology - gram negative bacilli & cocci
	I have an isolate from a blood culture which is a gram negative bacilli. Oxidase and catalase negative. Grows in CO2 no growth anaerobically. Growth on chocolate but not macconkey agar. Utilizes no sugars but urea positive. Neither fermentative nor oxidative. Can you help? Thank you (answered 05/25/2007)
	I have isolated a gram negative bacillus, oxidase negative, catalase negative, onpg positive, grows in CO2 and anaerobically, glucose positive, maltose positive, sucrose positive, trehalose positive, nitrate negative, esculin negative mannitol negative, ornithine negative, lysine negative, arginine negative, no growth on macconkey and non motile. What is it? More information - vitek 2 identified this as Sphingomonas paucimobilus but it is esculin negative. Grows on blood agar and chocolate well at 48 hours. Isolated from the blood of a cancer patient. Thank you (answered 05/17/2007)
	can Klebsiella oxytoca produce green sheen on EMB agar? thanks in advance..! (answered 05/01/2007)
	I have a slow growing gram negative rod I cannot identify. The gram stain showed long gnr with tapered ends so I began to take the Capnocytophaga route. All roads lead away from that organism. The isolate is catalase, oxidase, and indole negative. It doesn not grow on Mac. It doesnt exactly swarm the agar, rather it reminds me of what an Eikenella looks like, but on a longer scale. I have tried a few ID systems and it does not come close to any ID. Help? (answered 04/27/2007)
	i have a gm-ve isolate from et tip in my lab which is oxidase -ve, indole -ve, all sugars -ve , motile ,urea -ve, citrate-ve tsi k/a which is declared alcaligens spp by api on basis of id 32 i,m confused tell me what it is? (answered 04/27/2007)
	We had isolated a nonfermentative bacterium which shows a lemon-yellow nondiffusible pigment.After 48hrs of incubation at 30oC, a greenish pigment could also be seen.we suspect it to be Agrobacterium yellow group.Since we rely completely on manual methods of identification, what are the possible features for identifying this organism, or does any other nonfermenter produce this peculiar pigment(a mix of lemon-yellow and green)? (answered 04/26/2007)
	I would very much like to get a complete textbook/atlas on nonfermentative gram negative bacilli.Kindly help me in the search. (answered 04/26/2007)
	Is there any value in speciating Acinetobacter lwoffii from Acinetobacter junii? Is this important for determining antibiotic susceptibilities? (answered 04/21/2007)
	can I report shigella as final report with wellcolex by remel (answered 04/12/2007)
	Are there any other Hemophilus besides influenzae that are spot indole positive? Can we presumtively Id. an organism as H. influenzae if it is spot indole positive and call it Hemophilus species if indole negative? (answered 03/07/2007)
	Is it okay to incubate MacConkey II agar in CO2? I am concerned with a limitation listed in the MacConkey II product insert that states not all Enterobacteriaceae/Pseudomonas species will be recovered. Andera3@labcorp.com (answered 02/14/2007)
	i would love to know for certain if this is a pseudomonas aeroginosa. It is catalase +, oxidase +, glucose -, 6.5% nacl -, mconkey agar -, bile +, aerobic, g- rod, novobiocin - thyoglycate - (answered 02/06/2007)
	I am looking for a simplified flow chart for biochemical identification of medically important gram negative isolates. Biochemical tests curently in our laboratory are Indole, urea, TSI, manitol, motility, citrate and lysine. (answered 02/02/2007)
	On page 3.9.1.9 of the ASM Culture Procedure Handbook, under procedure section 4, the book states: "Identify the following only if the specimen......" and is followed in subheading b(1): "enteric gram negative rods (exception: Enterobacteriaceae are part of the normal microbiota......)". Could you please clarify, which enteric gram negative rods are not part of Enterobacteriaceae? (answered 12/11/2006)
	What are possible bacteria that is a gram negative rod that's oxidase positive, catalase positive, citrate positive, positive for nitrate reduction test, indole negative, MR negative, VP negative, urease negative, casease test negative, non-spore forming, non-acid fast non fermentative bacteria that's also non fermentative. It caused ropiness in the litmus milk test.. could it be Alcaligenes viscolactis? (It's not cited in Bergey's) (answered 12/11/2006)
	Can E. coli be indole negative and What kind of serotypes of E.coli is Indol-negative? (answered 11/21/2006)
	On day 1 a isolate Pseudomonas resistant to all antimicorbials except imipenem was detected in an icu patient. On day 3 the identification changed to E. coli with same AST result. The specimens were labeled retroperitoneal abscess on both the occasions. Kindly explain this discrepancy. (answered 10/17/2006)
	Is it OK to perform an antigen ID on suspect colonies of Salmonell/Shigella using the Wellcolex Colour Salmonella/Shigella kit and if the results are positive, it would be biochemically identified with a microscan panel. This way an entire day can be saved when results are negative by the Wellcolex method. Also, can both be charged for since one is a serogrouping and the other is an identification? What would be the appropriate CPT codes? (answered 11/27/2006)
	it's possible that P. aeruginosa is responsible of post-antibiotic diarrhea ? (answered 11/27/2006)
	Can Omnicef be used to treat pastuerella from a dog/cat bite? (answered 10/09/2006)
	I have isolated gram negative bacilli a late fermentor of sucrose and lactose and did nt produce a metallic sheen on EMB and neither did it answer any of the tests for the Enterobacteriaceae members, but is found to be oxidase negative, citrate and catalase positive and TSI showed acid butt and acid slant. Thanx a million if u can suggest me the possible organisms with the above features (answered 09/22/2006)
	got one isolate from wound swab which is oxidase positive,citrate also positive, but on AST colistin is coming resistant.is this not pseudomonas (answered 09/13/2006)
	Importance of N. meningitis in sputum cultures? (answered 09/12/2006)
	Is Haemophilus aegypticus still considered a valid name for Haemophilus influenzae isolated from the eye? (answered 08/30/2006)
	I have isolated a gram negative lactose fermenter which is indole and methyl red positive, citrate negative, urease positive and actively motile organism. Triple sugar iron agar shows acidic butt and alkaline slant with no gas and no Hydrogen sulphide production. can u help me (answered 08/10/2006)
	I have isolated a variant from my stock strain of E. coli that does not produce the characteristic green sheen on EMB, but keys out as E. coli on the Enterotube. The test results on the Enterotube are identical to my stock strain with no atypical results. It appears as E. coli on other media as well, such as MacConkey, SS Agar etc. I'm pretty sure it's E. coli. Any suggestions for additional, definitive tests to confirm that it is E. coli? If it is, is this a common mutation/variation in E. coli? Many thanks! (answered 07/15/2006)
	I got some isolates which give greenish metallic shean in EMB agar. They are indole -, MR +, Citrate + urease +,TSI acid butt and slant. They are gram negative rods. what can they be? (answered 06/07/2006)
	sensitivity pattern of Burkholderia spp and Sphingomonas spp ? (answered 05/26/2006)
	It has been our policy to report an indole negative Proteus that is ampicillin resistant as Proteus penneri. Such isolates were rare in the past, but recently we have seen an increase in occurrence. Given that Proteus mirabilis is now being categorized with E. coli and Klebsiella for ESBL production, we wonder if we need to rethink our policy. (answered 03/30/2006)
	What is the best way of Staph.stricking for factor- v. on blood agar satalitism on csf, is it the primary pool or at the secondary pool ? (answered 03/30/2006)
	What is the best way to store N. gonorrhea that we use for QC. Ours dies and we have to buy new ones. Any suggestions? (answered 03/03/2006)
	how come gram negative bacteria do not sporulate (answered 02/28/2006)
	gnc from a ventriculostomy, oxidase neg, cat pos, non motile, no growth on Macconkey, ng on BA anaerobically (answered 02/21/2006)
	What is the correct format for reporting Salmonella ANTIBODY results when serology is done? I have seen 2 different formats from other labs: 1. Salmonella O, Type D OR 2. Salmonella Type O, Group D Thanks for your help! (answered 02/09/2006)
	How reliable is the Vancomycin 5 sensitivity for differentiating gram variable rods? Is there a minimum zone size for calling an organism gram positive? (answered 02/01/2006)
	Can Neisseria cenerea have dry gry flat colonies on blood agar plate? can I get more information on this organism? Thanks. (answered 01/09/2006)
	I received the following Salmonella serum antibody results (EIA): Salmonella H, Type A Equivocal Salmonella H, Type B Positive Salmonella H, Type D Positive Salmonella O, Type VI Positive Salmonella O, Type D Negative How do I interpret this? Can I relate the results to a specific species/strain? Is there a chart for reference? Thanks for your help! (answered 12/19/2005)
	What is the correct format for reporting Salmonella ANTIBODY results when serology is done? I have seen 2 different formats from other labs: 1. Salmonella O, Type D OR 2. Salmonella Type O, Group D Thanks for your help! (answered 12/13/2005)
	How common is it for Neisseria gonorrhoeae to grow on blood agar? I always thought this organism was *strictly* fastidious. But I now have one that grew quite obviously on 5%SBA at 48hrs. It also grew up on the chocolate and in the Jembec system and both have been positively ID'd by our reference lab. (We are a POL- our county in currently experiencing a GC outbreak). Thanks in advance, Paula (answered 12/05/2005)
	is it possible to do molecular characterization of salmonella infection associated to human and animal .please help me in proceder ;thaks (answered 12/02/2005)
	Hello. To detect MRSA from a urine sample, is 48-72 hours considered an acceptable time for culture results? I have seen this time range in several publications but I don't know where it originates. Who sets the standards/guidelines in terms of turnaround time for MRSA detection and reporting? Does ASM? CDC? JCAHO? DHHS? Lastly, using the conventional techniques for isolating MRSA, would more than 100 hours to report a finding of MRSA be considered within the range of normal reporting times? Finally, is the lab that detects MRSA, after it notifies the physician, required also to notify nurse in charge of patient, or is this optional? Is the lab required to advise anyone other than the attending physician that a patient has confirmed MRSA? Thanks a million for your help! (answered 12/02/2005)
	What is Citrobacter Koseri & how is it contracted? (answered 10/31/2005)
	Does Salmonella arizonae type with the somatic antisera for Salmonella. In our clinical laboratory, we have the poly A-E, Vi as well as each individual antiserum. We recently had Vitek identify an organism from blood culture as 99% Salmonella arizonae and it did not type with our antisera. Thanks. Linda (answered 10/28/2005)
	I am interested in finding out more about Enterobacter sakazakii in stool cultures in other then neonatal patients. Is it a pathogen? (answered 10/19/2005)
	what is E. brevis (answered 10/05/2005)
	Is it possible to have an E.coli that doesn´t grow on McConkey? Both, an api 20E and Vitek GNI+ identified it as E.coli. Thanks. (answered 10/03/2005)
	Can Burkholderia cepacia strains have colonies with a metallic pinkish sheen? Since it is a slowly growing organism, is it possible to obtain different susceptibility results from Vitek if you do the testing on day 1 vs day 2 of growth? Thanks for the help. (answered 09/28/2005)
	I know that some other hopital labs are testing Hemophilus with the B-lactamse disk instead of the Kirby Bauer. What does the B-lactamse replace and what will the positive or negative result tell the doctors? Is this method acceptable? I would like to change if so. But before I do I need documentation that this method will be OK to use. (answered 09/02/2005)
	IS THERE A NON LACTOSE FERMENTING E. COLI? WE IDENTIFIED TWO ISOLATES AS E. COLI BY MICROSCAN THAT WERE INDOLE POSITIVE, BUT THEY DO NOT LOOK OR SMELL LIKE A TYPICAL E.COLI. (answered 08/29/2005)
	Is it sufficient to identify aeromnads to the genus and give a generic identification of Aeromonas hydrophila complex, provided that Vibrio, plessiomonads anf Chrombacterium speceis has been rule-out? (answered 08/17/2005)
	How would you identify Kingella kingae? (answered 07/26/2005)
	We are a small hospital and are looking for a cost effective way to definatively id Escherichia coli in urine cultures. We previously were doing a spot indole test on non-mucoid, lactose fermenters. We were told at our latest state inspection we have to report those as presumptive E. coli. Is a panel id the only way to go for a definitive id? We do not have a Microscan or Viatech, sensitivities are done Kirby-Bauer method. Thanks for your help. (answered 07/01/2005)
	Could a symptomatic patient have Bucella melitensis in his blood and NO simultaneous corresponding antibodies? (answered 06/30/2005)
	We isolated a GNB from a hand cellulitis. We could not identify nor could our reference lab. They sent it to Focus who id'd it as "EF-4". Could you give me any more information? What does EF stand for and what is the clinical significance? (answered 04/25/2005)
	I am trying to ID a G neg organism. I first thought it was Proteus because it grew purple on the EMB plate but my biochem results are not looking like Proteus. It is urease negative. Are there many G neg organisms that appear purple on an EMB plate? Thank you for your time. (answered 04/22/2005)
	is there a web site that i may lookup rapid identification for enterobacter (answered 04/18/2005)
	In this era of cost slashing is it necessary to continue to add a chocolate plate to specimens ordered specifically for G.C.? Or will a selective G.C. media such as MTM suffice? Thanks, Javier Garcia (answered 02/24/2005)
	What negative control organism should be used to QC Smith-Baskerville media for B. bronchiseptica (answered 02/22/2005)
	Haemophilius parainfleunzae in a 4 year old with chronic sinusitis. Should I perform Kirby-Baurer testing on this organism (answered 11/05/2004)
	Does gonorrhoea use carboyhdrate, amino acids or co2 as a source of energy? (answered 10/19/2004)
	Neisseria meningitidis seems to be appearing more frequently as a predominant organism in respiratory cultures. I'm interested in ASM's opinion regarding prophylaxis of family members when the organism is recovered from the respiratory tract. (answered 10/07/2004)
	Is shiga toxin e coli a non fermentor or a non sorbital fermenter? (answered 10/07/2004)
	Eikenella coorodens: Are there reliable biochemical tests for it, or is it only a diagnosis by view on the plate? In our laboratory the VITEK II is used, but this germ is not in the profile, as there is the same problem with other germs of the HACEK-group. How are they analysed in routine clinical microbiology? Are there "Screening" reactions? Thank you (answered 09/27/2004)
	Which media is currently the most acceptable for isolating Bordatella bronchiseptica? (answered 08/13/2004)
	which selective media would be used to isolate Klebsiella oxytoca? (answered 08/13/2004)
	i'm looking for an organism with these clues: causes bacteremia, wound infection, gram-negative, curved rods, sensitive to tetracycline and fluoroquinolones, possibly acquired from swimming, some published reports of penicillin susceptibility. pls help. (answered 08/06/2004)
	What is the origin of N meningitis W135? Why is the serogroup named W135 ? (answered 06/25/2004)
	Current guidelines for culturing for Hacek organisms in blood cultures (answered 06/22/2004)
	Is e coli acid negative or acid positive and gas negative or gas positive in glucose, lactose and sucrose fermentation. (answered 06/22/2004)
	Is E. coli acid-negative or acid-positive in OF-glucose testing? (answered 06/22/2004)
	What is the correct protocol for setting up a urine specimen for G.C.? The MCM 8th. Ed. and CUMITECH on UTI's seem to imply that no modifications of the streak plate are necessary with the exception of adding a chocolate or selective GC plate. Would it not be necessary, espcially in the case of females, to centrifuge the specimen given the fact that there is lower sensitivity in recovery of GC when processing such a specimen? Should not a drop of this sediment be inoculated onto selective media rather than using a calibrated loop to increase the sensitivity?  (answered 05/04/2004)
	what is the path of salmonella when it enters the body, what cells does it infect in what organs, does it effect recetors etc (answered 04/27/2004)
	What is the best method for screening for Bordetella pertussis? We now set up a culture using Regan-Lowe Charcoal media and a BAP plate and send off to a reference lab for DFA. I have seen literature on a Bordetella pertussis antigen. How does it compare to the culture and DFA and who is it availabe from?  (answered 03/15/2004)
	What are the definitive tests for identifying H. pylori in a culture? (answered 02/13/2004)
	Are all isolates of E.coli 0157:H7 spot indole positive? (answered 02/10/2004)
	i'm a micro student currently testing a multi med the bbl oxi/ferm tube II i have no biocode manual available to look up the numbers 44713. i'm having trouble id the final result i've searched the web for a ready available manual with no sucess. please help (answered 11/12/2003)
	What is the pathogenesis and role of fever in Typhoid Fever? (answered 10/29/2003)
	Is there any real advantage of using a MUG test as opposed to a PGUA/Indole test on non-lactose fermenting E. coli? (answered 10/15/2003)
	What percent of Proteus mirabilis isolates are non swarming? (answered 08/11/2003)
	Is acinetobacter lwoffi a pathogen in the eye? (answered 07/22/2003)
	I am looking for disease with these test criteria. Gram negative rod, facultative anaerobic, negative oxidase, ferments glucose. negative H2S production, negative Indole, positive VP, positive citrate, positive catalase, positive NO3 reduction, lactose glucose and sucrose are acid gas positive. I have narrowed it down to Enterobacter aerogenes but have not found information that is a conclusive answer. What do you think? (answered 07/15/2003)
	We are using an Alpha streak to identify Gardnerella vaginalis, I can't find any literature to support this test. My procedure manual references an ASM JCM article from the late 80's. Is there anyway I can get a copy of this article for using an Alpha streak to presumptively id Gardnerella vaginalis? (answered 05/13/2003)
	are the colonies for e.coli 0157 lactose or non-lactose fermenter on regular macconkey? (answered 04/23/2003)
	Our MicroScan Alert software flags all sorbitol negative E.coli as possible E.coli 0157 - regardless of the specimen source. Has E.coli 0157 been isolated from any specimen other than stool (i.e. is it necessary to rule out E.coli 0157 when the source is urine? How about if the source is blood?)? (answered 04/07/2003)
	IS O129 VIBRISTATIC AGENT A USEFUL TEST FOR VIBRIO CHOLERAE IDENTIFICATION? (answered 03/27/2003)
	Are there any reports of Campylobacter jejuni isolates from stomach biopsy specimens that were initially worked up as "suspect" Helicobacter pylori? (answered 03/03/2003)
	If an E coli appears as two different organisms, but has the same susceptibility and biotype # (Vitek), should they both be listed, or is it really just one organism? (answered 02/19/2003)
	Can E. coli be indole negative, MUG negative and nonmotile? (answered 02/18/2003)
	Are the following organism names current or are there more appropriate names: Enterobacter taylorae Enteric group 45 CDC group IV C2 Koserella trabulisii Leminorella species (answered 02/14/2003)
	A recent CAP survey D-11 summary advises "two confirmatory tests on suspicious isolates from any clinical site...If a laboratory only has one confirmatory test...the isolate should be referred". Some of my colleagues interpret this to mean any GC isolate must be identified by two different methods, not just those from children, sexual abuse cases, or non-genital sites, as I had always understood. Please clarify current requirements for GC identification from genital and non-genital cultures. (answered 01/17/2003)
	WE HAVE RECENTLY IDENTIFIED AN N. GONORRHOEAE, USING THE API NH SYSTEM, THAT WAS HYDROXYPROLYLAMINOPEPTIDASE NEGATIVE. IS THIS POSSIBLE? (answered 01/10/2003)

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