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Culture, genital
	If a group B streptococcus is recovered from high vaginal swab for non pregnant lady should we continue for sensitivity and reporting or consider it normal colonization and is E coli a vaginal pathogen to report (answered 06/20/2007)
	Should we evaluate vaginal swabs for atrophic vaginitis in post menopausal women and desquamative inflammatory vaginitis and non infectious vaginitis in all women? (answered 06/20/2007)
	IS HEAVEY GROWTH OF GROUP F BETA STREPTOCOCCUS IMPORTANT IN A VAGINAL (answered 05/11/2007)
	SHOULD 4+ STAPH AUREUS IN GENITAL CULTURE HAVE A SENSITIVITY PERFORMED? (answered 05/07/2007)
	Our lab is updating the genital culture. We are debating to get rid of the gram stain. Should the gram stain be ordered for the diagnosis of Bacterial Vaginosis? Or should BV be idenitified from the plate? (answered 04/24/2007)
	We do a few genital cultures still, and the physician is good about including the diagnosis. But I've been concerned about the situation in which the genital smear suggests bacterial vaginosis (no Lacto, many small variable rods and rare yeast), but then the culture grows many yeast. We then feel obligated to ID the yeast, but actually the gram stain suggested yeast was a minor presence. When the gram stain shows rare yeast, should we limit our yeast workup? Possibly report as "Many yeast, no further ID indicated by original smear." In this case, the dx was polycystic ovaries. Thanks. (answered 04/17/2007)
	What is the significance of Gardnerella vaginalis in male genital cultures? Should this organism be reported, and is it a cause of UTI in males? Thanks (answered 04/17/2007)
	How should a negative (no pathogens) genital culture report (not a GBS screen) be worded? Can you include the statement "No Gp B Beta strep isolated" if you did not set up a LIM broth on these cultures? Should LIM broths be set up on all genital cultures if physicians sometimes order a genital culture instead of a GBS screen? (answered 04/05/2007)
	is gardnerella vaginalis normal vaginal flora? (answered 01/31/2007)
	What is clinical importance of Streptococcus pneumoniae in urethral swab? (answered 11/14/2006)
	We follow the guidelines for Group B Strep screenings for pregnant patients using LIM broth. We only test beta hemolytic colonies for Group B. Recently we had a culture that the tech tested because he thought the colonies looked suspicious. They were indeed a nonhemolytic Group B. We are now concerned that we could be missing these as per our protocol we only ID hemolytic streps. Is this a real concern and if it it, is there a cost effective way to test for nonhemolytic Group B? (answered 10/30/2006)
	In your opinion, what should be worked up in genital cultures during pregnancy ? If the diagnosis is just pregnancy, shouldn't they just be ordering the beta strep B screen? We have a couple physicians that screen for E.coli. Is this appropriate? Thank you (answered 10/24/2006)
	Should Enterobacteriae group of organisms be reported in vaginal swab? If commensal flora is not isolated in vaginal swab clture, what does that indicate? Thanks (answered 08/10/2006)
	What microorganisms must we look for in a study of infertility in a woman? (answered 06/02/2006)
	Which are the organisms significant in a High Vaginal Swab? How will we identify G.vaginalis in clinical laboratory (answered 05/15/2006)
	I'm a veterinarian, submitting vaginal smears to a lab, for bacterial culture and sensitivity.What we usually do in the clinic is we put the swab in a sterile tube with nutrient agar. But just recently the lab gave me chocalate agar media. They told me to directly put my specimen in it.. I want to know the significance of this chococalate agar in culturing samples coming from the genital area. And can't samples from vaginal smears grow in regular nutrint agar? (answered 05/15/2006)
	may i have the list or standard operating procedure in collecting and culturing vaginal discharges? Please include the different media to be used as well as their significance.. thanks (answered 05/15/2006)
	Aside from known genital pathogens (i.e. GC, Chlaymdia, H. ducrey ) what other organisms should be worked up from routine genital cultures? Specifically should any gram negative rods, yeast, beta strep, G. vaginalis or Staph aureus be worked up and to what extend? (answered 02/28/2006)
	I just had a vaginal culture which had a moderate growth of haemophilis parainfluenzae and noral urogenital flora, so what is the cause? Is it contagious? If left untreated what would happen? Should my boyfriend take the ampillin as well? (answered 01/26/2006)
	I have information that there are some posters demonstrating carryover nucleic acid contamination from GC/CT when PCR is done after cytologic preparations are made. Please I need to find out that information. Thanks. (answered 11/21/2005)
	For genital cultures, we are wondering if it would be appropriate to bill for a Gardnerella screen (code 87081) along with code 87070. We are currently using five plates for our genital cultures: Chocolate, MacConkeys, Blood, GC-Lect, and HBT-Bilayer to check for Gardnerella. The HBT-Bilayer is quite expensive, and we are wondering if reimbursement for this is a possibility. Thanks. (answered 10/28/2005)
	BIO BAG C WITH MTM PLATE is this necessary if putting in CO2 incubator (answered 04/27/2005)
	I am trying to find a current procedure for doing wet prep on rape,children and general patients that come through the er. We are incorporating the gram stain into this for bacterial vaginosis but, still would like to know what is appropriate to answer directly off of the wet mount. (answered 09/17/2004)
	what is the incubation time for a genital culture using selective media (looking for Neisseria gonorrhoeae)? (answered 09/17/2004
	EEC - what is an endocervical curotage? (answered 09/13/2004)
	Is it true that CLIA will be making a move soon towards requiring that chlamydia nucleic acid amplified tests be run with amplification controls? (answered 08/13/2004)
	My question is concerning screening for Group B strep on pregnant women, CDC recommends incoulating a plated media and also to include a broth media such as LIM broth or Todd Hewitt with antibiotics. If the originial plated media from the specimen does not show evidence of Group B strep and is not overgrown with gram negative rods, just other usual flora - enterococci, Coagluase neg. staph., lactobacilli, and the like, is it necessary to subculture the broth media to plated media? If that subculture then has Group B strep, is this low number of strep significant enough to cause streptococcal infection in newborns? (answered 08/02/2004)
	WHAT IS THE BEST(RECOMMENDED)WAY TO PROCESS THROAT SWAB, THE MEDIA USED, THE CONDITION AND THE TIME?  (answered 05/17/2004)
	What is the procedure for a KOH prep on a vag/cervical specimen? (answered 02/12/2004)
	can repeated miscarriages (when no other apparent medical cause exists)be result of a pathogenic microorganism (answered 02/11/2004)
	Should WBCs seen on gram stains from centrifuged fluid specimens be semi-quantitated? And what is considered normal vaginal flora from women over age 55 and what would be considered pathogens?  (answered 10/30/2003)
	Recommendations regarding GC transport systems and have their been any comparisons between the JEMBEC and the Amies agar with charcoal transport swab in viability and recovery of the organisms within 24 to 48 hours.  (answered 10/22/2003)
	What is the appropriate procedure to collect, transport and interpret a vaginal wet prep? Are there any references to consult for this procedure? (answered 05/01/2003)
	What is the appropriate procedure (and references) for performing a wet prep on vaginal specimens? What is the appropriate transport container and conditions? How does one determine the percentage of clue cells in the wet prep?  (answered 05/01/2003)
	Do you add blood supplement to the Transvag media for isolation of GBS? (answered 03/21/2003)
	Why isn´t the Biomeriéux Mycoplasma IST used in the US for the detection of genital Mycoplasma and/or Ureaplasma? (answered 03/14/2003)
	Culture,genital: What is the best way to detect Neisseria gonorrhoeae in clinical specimens?  (answered 03/03/2003)
	What is the most sensitive and specific method for diagnosing bacterial vaginosis? How does the gram stain and applying the Nugent criteria fare in this regard? (answered 02/28/2003)
	What are other labs doing in re: working up GNRs in genital cultures? Are they being considered as part of the normal flora and hence not mentioned, or are they being quantitated and reported? If so, at what enumeration is a susceptibility being performed? Or is one not performed? (answered 02/24/2003)
	What organisms should be reported from "routine vaginal cultures"? We consider enteric gram negative bacilli and enterococci as part of normal vaginal flora and do not report them separately-isn't that correct? What about hemophilus influenzae? I am concerned about reporting too many organisms and having physicians treating patients for " normal flora." (answered 02/24/2003)
	What is the significance of working up gram negative rods in female genital cultures? Do most places mention them in the report, or consider them normal flora? If mentioned, in what amounts (ie. moderate? abundant?) would a susceptibility be performed? (answered 02/24/2003)
	How are laboratories handling Hemophilus ducreyi cultures? Are most simply not culturing for it? What methods are available to help a physician make a diagnosis, and which is best? (answered 02/07/2003)
	What is the current opinion for prevention of Group B Strep infection in newborns for a GBS postive mother? (answered 07/11/2002)

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