Culture,
stool
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Could you tell me the significance of isolating Klebsiella oxytoca from a stool culture? When should this organism be identified and reported from a stool culture? Thanks!
(answered 06/17/2007)
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Is there an recent paper available comparing various EIA kits for C. difficile toxin A and B?
(answered 06/13/2007)
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What is the recommended frequency for performing cdiff toxin assays? We often get multiple samples collected on the same day. Are there recommended quidelines to follow?
(answered 05/20/2007) |
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WHEN THERE IS NO GROWTH ON MEDIA PLATES,IS IT OK TO REPORT NO GROWTH ON STOOL CULTURE INSTEAD OF REPORTING NO SALMONELLA, SHIGELLA, E.COLI O157:H7 AND CAMPYLOBACTER ISOLATED?
(answered 05/12/2007)
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Is it clinically relevant to test for C diff toxin in patients who are currently receiving either Vancomycin or Metronidazole?
(answered 04/25/2007)
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How important is it to place stool material for culture into transport media if there is a delay in plating the samples? We currently refrigerate the samples if there are delays in plating? Is the costs of tranport media justified vice refrigeration? Also, the guidelines are confusing on temperature?
(answered 04/23/2007)
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How long will C. difficile toxins be detectable in the stool after a patient is treated for C. difficile? We use a EIA kit test to detect the A and B toxins.
(answered 04/21/2007) |
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What is the recommended method of reporting leukocytes in stool? What is the recommended stain to use? What is considered normal? Thanks, anewman@olbh.com
(answered 04/15/2007) |
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I am currently considering a test for shigatoxin. One of the tests differentiates shigatoxin 1 and 2 and one does not. Is it clinically significant to differentiate between the two?
(answered 03/19/2007) |
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Is a pure culture of Hafnia alvei significant in a stool culture? How should we report it? We should not report susceptibilities, correct?
(answered 02/09/2007) |
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Is a diaper from a child an acceptable specimen for c.difficile testing?
(answered 10/20/2006) |
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WHAT IS THE BEST WAY TO CULTURE STOOL FOR C.DIFFICILE?
(answered 07/16/2006) |
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We occasionally get requests for S. aureus cultures on stool. Should we not offer this as a test since the results could be misleading?
(answered 04/20/2006) |
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We currently only set up a MacConkey, XLD and Campy agar for routine stool cultures. Should we add a BAP?
(answered 03/29/2006) |
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What are some causes of "no growth" stool samples- besides antibiotics, improper collection and handling? Please include any medications or chronic disease.
(answered 03/14/2006) |
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Is there any cross reactivity with C. difficle toxin when testing for shiga toxin with the ProSpecT EIA kit? Can you cite articles/references? Thank you.
(answered 01/23/2006) |
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SHOULD WE BE CULTURING FOR CLOSTRIDIUM DIFFICILE AND PERFORMING A SUSCEPTIBILITY?
(answered 12/20/2005) |
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WE WERE REVIEWING THE LASTEST ASM GUIDELINES FOR STOOL. WHAT IS DF-3 AND HOW DO YOU IDENTIFY IT. ALSO, IF FOUND, IS THERE ANY SUSCEPTIBILITY TESTING THAT NEEDS TO BE DONE?
(answered 12/08/2005) |
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"Is it appropriate to use a PYR positive result to rule out Salmonella and Shigella in stool isolates? May this spot test be included in a stool culture SOP?
(answered 10/05/2005) |
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How many tests for c-diff need to be done if 1st one negative but c-diff suspected as cause of diarrhea?
(answered 08/22/2005) |
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What are the current recommendations for the reporting of Aeromonas and Pleisiomonas in stool cultures? Should they be reported whenever isolated, or only when present in predominant numbers?
(answered 08/12/2005) |
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when I examined a Gram-stained preparation of a stool sample from 25 year old man, I found a lot of (more than 10 bacteria per each field)organisms resembling Borrelia. They had similar morphological characteristics with Borrelia species. Is that possible to observe such organisms in a feces sample? what would be the interpretation?
(answered 07/20/2005) |
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Where can I find a procedure for "Quantitative Duodenal Contents Culture"?
(answered 07/11/2005) |
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SHOULD WE BE DOING GRAM STAINS ON STOOLS?
(answered 06/23/2005) |
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We received an order for a quantitative culture of small bowel contents. Is there a reference I can use to write a procedure?
(answered 06/09/2005) |
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Can we report Campylobacter sp. on stool cultures or do we need to distinguish between jejuni and coli? If we do need to speciate, what is the best test to use to easily do this. We currently use a nalidixic acid disc and if it is sensitive, we report C. jejuni.
(answered 04/27/2005) |
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Hi, our microbiology currently look for C.diff toxin a/b using both toxin
testing on faeces and on cdiff colonies. For patients shown to be positive
in the last few weeks and are being repeatly tested/requested for toxin
analysis, we just look for toxin on colonies. Seems longer, but cheaper.
What is the current thinking on 1) the best way of detecting toxin A/B and
2) the most realistic way of dealing with the constant requests on patients
that have tested positive in recent weeks
(answered
03/02/2005) |
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what is the recommended incubation time for the recovery of
campylobacter from stool using campy cva media and 42 degree incubation.
(answered
02/10/2005) |
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A
stool specimen returned results with aeromonas cabiae listed. What is this?
(answered
11/19/2004) |
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IF YOU HAVE A STOOL CULTURE THAT HAS ONLY ONE ORGANISM OF GRAM NEGATIVE
FLORA (SUCH AS E. COLI OR PSEUDOMONAS)AND ALSO SOME GRAM POSITIVE FLORA,
IS THE GRAM NEGATIVE ORGANISM CONSIDERED A POSSIBLE PATHOGEN? THE
CULTURE GREW 2+ GRAM POSITIVE NORMAL FLORA AND 2+ E. COLI (NEGATIVE FOR
E. COLI O157:H7) AND NO OTHER GRAM NEGATIVE FLORA. OUR DOCTOR WAS UPSET
THAT A SENSITIVITY WAS DONE ON THE E. COLI.
(answered
11/12/2004) |
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How do I explain to a physician why stool mixed with urine is not
acceptable for culture?
(answered
11/11/2004) |
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Should we still be using a gn broth as part of our routine stool culture
protocol? I have never found that it has helped in isolating Salmonella
or Shigella. They are found on the primary plate or not at all. It seems
like a waste of time and money. I also note that there are no
reimburements for using broths via CPT codes.
(answered
10/27/2004) |
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Are there criteria for performing stool cultures on patients that
develop diarrhea >3 days of hospitalization and are C.difficle toxin
negative in multiple specimens?
(answered
10/22/2004) |
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I have recently been informed that the differention of Campylobacter no
longer includes the use of antibiotics, Nalidixic acid and Cephalothin.
Is this correct? What are the current guidelines for species
identification?
(answered
10/21/2004) |
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How many different serotypes are of Salmonella are currently identified?
(answered
10/15/2004) |
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RE neonatal rectal swab from NICU. How do you interpret the cultures. How
fast does the rectal area become colonized after birth?
(answered
10/06/2004) |
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In patients with acute diarrhea, are there references or a consensus of
opinion as to when, due to duration of the illness, stool cultures may
not be cost-effective (appropriate?)because of low yield?
(answered
10/01/2004) |
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My question is regarding fecal leukocytes. I get requests for this
-mostly from pediatricians or gastroenterologists. Our current protocol
recommends that testing be done within 4hr but most samples are received
from satellites labs and are delayed in transit. I am trying to figure
out the best approach in dealing with this. Would a slide made at the
referring lab (equal aliquots of saline and methylene blue plus small
amount of stool spread onto a slide) and then forwarded to us be
adequate for testing? I have also read about a test called lactoferrin
which is more stable but I don't know much about it. Thankyou for your
time and expertise-this is a wonderful resource!
(answered
09/24/2004) |
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Can any fecal leukocyte testing be done from a Cary Blair vial?
(answered
09/08/2004) |
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Can you please provide some guidelines for health care providers
concerning when it is most useful to order stool WBCs, and when it is
not? Thank you.
(answered
08/31/2004) |
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WHAT IS THE RECOMMENDED FREQUENCY FOR TESTING CLOST. DIFFICILE TOXIN?
(answered
08/05/2004) |
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When testing for C. difficile toxin, is it necessary to test for both A
and B toxin?
(answered
08/03/2004) |
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Is
it necessary for the clinical lab to serotype Shigella,since these
isolates are sent to the state lab?
(answered
04/30/2004) |
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What
is the best way to culture for Aeromonas in routine stool cultures?
Currently, we only culture routinely for Salmonella, Shigella and
Campylobacter; E. coli 0157 by request or if the specimen is visibly
bloody; Yersinia and Vibrio by request. We do not set up a BAP but we do
set up a CNA on our primary plating media along with a HE, XLD and Campy
agar as well as selenite broth. Is Aeromonas a common stool pathogen,
enough to routinely screen for it? Should we be setting up a BAP instead
of a CNA plate? I cannot find any really good resources on Aeromonas as a
potential stool pathogen and isolation procedures.
(answered
04/26/2004) |
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re
isolation of campylobacter from stool specimen: Is the double enrichment
process still necessary. We only do a single enrichment. We incubate the
innoculated alkaline peptone water overnight then we subculture to TCBS.
Are following best practice or haave we developed bad habits
(answered
04/26/2004) |
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What
method is preferred for toxin testing of Clostridium difficile. I know
that the newer EIA test kits are very sensitive and specific. Is there any
advantage to performing the toxin assay by cell culture vs the EIA? Any
references would be helpful in making the determination.
(answered
04/20/2004) |
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our
lab collectively reports lactose fermenters as "coliforms" in
stool cultures. We also enumerate Proteus, Pseudomonas, S. aureus, and
yeast if present in moderate or large numbers. Is this an appropriate way
to report stool cultures? We also comment on the absence of fecal
pathogens.
(answered
11/11/2003) |
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Over
the past six months our lab, located in southern Ontario has isolated
Edwardsiella tarda from three separate cases of hosptialized patients with
acute diarrhea as the only potential pathogen. Previous to this the last
E. tarda was 18 years ago. Are any other labs seeing an increase in their
E. tarda isolates? Do labs routinely report this isolate if isolated from
stool samples?
(answered
07/31/2003) |
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If
Bacillus is found to be predominant in a stool culture, should it be ID'd?--Could
it be cereus food poisoning? Also, what is the significance, if any, of
predominance of Kleb sp. in stool cultures?
(answered
06/10/2003) |
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What
is the significance of finding Bacillus spp. in stool cultures--especially
if predominant? Does one worry about cereus food poisoning?
(answered
05/23/2003) |
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In
stool cultures that have no or very little normal fecal flora is it
relevant information to report large numbers of Staph aureus, Pseudomonas,
and yeast
(answered
05/05/2003) |
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Isn't
it clinically relevant information to report normal flora especially when
it is reduced or absent in cultures like sputums or stool. eg when there
is gram positive fecal flora only (no enterics present)in a stool culture,
or when normal respiratory flora is reduced or absent.
(answered
05/05/2003) |
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Since
C. difficile has been shown to be present in up to 50% of asymptomatic
infants under one year of age, is it approriate to do c. difficile testing
on children in this age group? Our pediatricians seem to frquently order
these tests on infants. A related question, should formed stools be
rejected for C. difficile testing? What about multiple specimens collected
on the same day?
(answered
04/24/2003) |
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Is
it recommended that one perform a Clostridium difficle culture on stools
which are negative for Toxin A and B by EIA (specifically Meridian brand)?
(answered
03/14/2003) |
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What
species of Enterococcus should we report from surveillance cultures
(rectal cultures) for VRE other than Enterocccus faecalis and Enterococcus
faecium? I realize we should exclude those intrinsically resistant species
with the vanC genotype.
(answered
03/11/2003) |
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What
is the recommendation when testing for C.dificile toxin-A, B or Both?
(answered
02/28/2003) |
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Is
there a routine cost-efficient commercial lab test to detect Shiga toxin (Verotoxin)-producing
E. coli (STEC [VTEC]) non-O157 (such as O26, O103, O111) in bloody or
watery stool specimens, since these non-O157 strains may represent a
significant proportion of STEC isolates in many parts of the U.S., Canada,
and Europe?
(answered
02/17/2003) |
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What
is the best method to recover Campylobacter spp. from swabs?
(answered
08/15/2002) |
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Is
there currently a standard method for the detection and enumeration of
Salmonella?
(answered
08/15/2002) |
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Which
agar is best suited to recover campylobacter spp. from enteric swabs...
What tests can be used to confirm the presence of campylobacter spp.
(answered
08/05/2002) |
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