Culture, urine

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Culture, urine

How do you calculate for urine colony count in cells per ml?

(answered 06/20/2007)

coryneform bacteria seen in gram stain of sputum culture and the coryneform gpb grows 4+. Should further id be done?

(answered 06/11/2007)

Looking at a Clean Catch urine sample and you have less than 10,000 col/ml of an organism we do not work it up, however, I have the question can you call this a no growth or should it be reported <10,000 col/ml with a description of the colony type?
(answered 06/11/2007)

could you get a positive nitrate reading on urinalysis but then get a negative urine culture if the patient was being treated with Levaquin 500mg for 6 days?

(answered 05/18/2007)

what is the sop for urine culture according to asm/ if 2-3 pus cells are there and growth is >100,000/ml so should i report and perform ast?
(answered 05/18/2007)

cystoscopic urine nephrostomy urines,how should we treat these cultures both in regard to plating and reporting.

(answered 05/16/2007)

Question about culturing of urines: We use NON-DISPOSABLE calibrated loops for plating urines. Is it necessary to flame the loop between plates (if the tech makes sure a drop of specimen is delivered)?

(answered 05/08/2007)

Is it acceptable to culture a urine specimen on a patient that is taking phenazophyridine (Azo-Standard)? If not acceptable, what time frame should you wait after the patient discontinues its use before culturing the urine? Thanks.
(answered 04/13/2007)

Is any one rejecting urines for culture if the dipstick was negative for leukocyte esterase and or nitrates?
(answered 04/01/2007)

Nitrite negative on original automated urinalysis but positive bacteria on microscopic, at what amount is the recommendation for setting up a culture?
(answered 02/19/2007)

What are your recommendations for working up foley/indwelling cath urine cultures with multiple organisms?
(answered 01/10/2007)

We receive a lot of clean catch urine specimens for culture from outpatients, OB department and ER department that contain a great deal of mixed flora. Is it still recommended to clean the area with a towelette before collecting clean catch specimens? What is the proper procedure so we can explain to our patients?
(answered 11/14/2006)

Is there a legitimate reason for doing a urine broth culure? Some of our docs recognize interstitial cystitis as a real syndrome but most do not.
(answered 10/26/2006)

I should like to know if there is some protocol that establishes as correct to process two specimens of urine taken in the same day (a few minutes apart) in pediatric patients, or if the correct action is to reject one of them. Thank you for your answer
(answered 10/12/2006)

Staph saprophyticus pyelonephritis. Is it possible? Have any blood cultures been found positive for? We have a hospitalist who believes she has a case and patient is going downhill.
(answered 10/12/2006)

on urine cultures that grow only mac is it acceptable to call it gram negative or do we need to perform a gram stain as well. we send the organism out for id/sens.
(answered 10/12/2006)

For interpretation of colony counts in urine cultures, can these be taken from MacConkey or CNA agars instead of BAP?
(answered 07/25/2006)

The latest URINE CULTURE procedue as issued by ASM in its Microbiology Procedure Manual states to workup (ID and AST) at =>1,000 CFU/ML single isolates from "women 14-30" (instead of =>10,000). Explain to me why this is so and why this is important to do. Please. Thanks. Mary Kulpa, Microbiology Supervisory, Wheeling Hospital.
(answered 07/25/2006)

For interpretation of colony counts in urine cultures, can these be taken from MacConkey or CNA agars instead of BAP?
(answered 07/25/2006)

have there been any reports of Pneumococci causing UTI
(answered 07/18/2006)

What is an "acceptable" contamination rate for clean catch urines? We have a lot of contamination based on our procedures and we are trying to work with nursing to obtain better quality samples. If you have any suggestions or ideas, it would be greatly appreciated. Almost every urine we get from ED and or OB is contaminated i.e >4 organisms or Normal flora only. Thanks
(answered 07/07/2006)

Is streptococcus viridans found in the urine @ 10,000-50,000CFU/mL related to a unproper clean catch? Should the specimen be recollected? Is this organism related to pregnancy?
(answered 07/06/2006)

For how long is it acceptable to read a urine colony count (primary plates)? Our laboratory works Monday thru Friday; for those colony counts inoculated on Friday, is it acceptable to read them next Monday or should we not perform urine colony counts on Fridays? Thank you
(answered 06/29/2006)

should Gram stains be made from spun or unspun urine specimens?
(answered 05/27/2006)

When do you report "Poor Collection" vs. "Probable Contamination" in a urine with multiple junky organisms (lactobacillus, alpha strep etc.)
(answered 05/12/2006)

The presence of GBS in the urine of a pregnant woman, in any number or count, and at any time during the pregnancy, does imply always the need of antimicrobial prophylaxis?
(answered 05/10/2006)

Is it necessary to do antibiotic sensitivity testing on strep viridans isolated from urine cultures?
(answered 05/10/2006)

What is thc significance of the "other" gram positive cocci (e.g. Aerococcus urinaehominis, Gemella, Facklamia, etc.)in UTI? The majority of patients we are isolating these organisms from are nursing home, from a straight cath, usually the only isolate (or one of 2), with a colony count of >100,000 and with a U/A that shows definite signs of infection (e.g. >= 100 wbc/field). Should these be considered pathogenic, or should they be reported out as skin flora?
(answered 05/09/2006)

Do you recommend including the urine sediment results (especially leucocites, epith. and bacteria)with the ID and sensitivity results of a urine culture? In other words, do you use the urine sediment just to verify the acceptability of the specimen or do you give the physician the information? What about the Gram stains from other type of cultures such as wounds and sputum? Is the Gram stain reported with the culture results or is it just used within the lab? Thanks, Loyda Oduber
(answered 05/02/2006)

We do our urine microscopy for WBC and RBC by oserving a drop of well shaken urine sample microscopically and reporting it as per HPF,could we report this finding per litre (or milliliter)also(by suitable calculation)? Please guide.Thanks,Ali.
(answered 05/02/2006)

URINALYSIS RESULTS WITH THE FOLLOWING: leuk esterase/ large; URINE MICRO with the results: urine WBC 5-10 ; bacteria 3+; transitional ep 2-5; squamous eptih 10-25; final report 25-50,000 col/ml mexed gram positive flora please explain if possible thanks
(answered 04/03/2006)

What are some reasons that you may have bacteria in a microscopic urine but the culture is negative?
(answered 03/30/2006)

In the Clinical Microbiology Procedures Handbook, 2nd edition, for urine cultures (3.12)it lists Corynebacterium (urease positive)in the uropathogens (Table 3.12-1). It states ID and AST in the workup. What antibiotics should be tested and what quidelines should we follow?
(answered 03/30/2006)

I have a very basic question about planting urine cultures. When using a calibrated sterile disposable loop, should you use a new loop between plates? I know many people that use the same loop. Thank you
(answered 03/21/2006)

Is Staph aureus considered to be a potential pathogen in the urine? Should it be worked up when it is found in equal amounts with normal flora?
(answered 03/07/2006)

what Cornyebacterium causes serious urinary track infections?
(answered 02/26/2006)

We are updating our urine culture procedure and have had some discussion on the classification of S.saprophyticus, S.xylosus and S.cohnii. Are these three organisms subspecies of the S.saprophyticus group, and is it necessary to perform susceptibility testing for high colony counts of S.xylosus and/or S. cohnii? Thank you for any information.
(answered 12/15/2005)

Our microbiology laboratory has just introducted a new urine procedure based on the ASM "Clinical Microbiology Procedures Handbook" second edition.Example, we use an .001 loop and a .01 loop depending on the type of urine collection. I have called several area microbiology labs and none of them are using this protocol. What is your thoughts we seem to be working up more positives? Is any one else using this procedure? It is always are aim to stay current with ASM procedures.
(answered 11/23/2005)

What is the significance, if any, of lactobacillus (100,000 col/ml) in urine cultures? Should we consider it a possible uropathogen or a possible skin contaminant? Would you recommend supplementing a report of 100,000 col/ml lactobacillus with a comment indicating possible contamination?
(answered 11/16/2005)

Should epithelial cells be reported on urine gram stains?
(answered 09/28/2005)

hello.please provide me a simple methodes to culture swab from urinary tract & what is normal flora present in urine swabs .thanks
(answered 09/28/2005)

Our lab processes urine for urinalysis and culture from the same wide mouth container (dipstick & loop). We have not experienced a contamination problem. Is this an acceptable practice? I have been unsuccessful finding literature to prove otherwise.
(answered 09/28/2005)

Is there a recommended method of performing culture of urinary calculi?
(answered 08/22/2005)

DO WE NEED TO REINCUBATE ALL NO GROWTH URINES AFTER 24 HOURS FOR ANOTHER 24 HOURS ? tHANKS.
(answered 07/29/2005)

Should we stop handing out benzalkonium towelettes for cleansing prior to midstream urine culture collection? Does the data support not cleansing?
(answered 07/29/2005)

We have a certain physician client who has standing instructions for us to "work up ANY growth, in ANY amount" on urine cultures. He is a FP/IM practitioner, NOT a uro- or nephrologist. He also routinely questions "No growth" reports, saying that urinalysis was strongly positive for bacteriuria at his office (3+ bacteria, 3+ Nitrate, etc.), and that the patient was not treated before testing. Upon first round of complaints, we even replated preserved urine on chocolate agar and anaerobic media to rule out anaerobes and fastidious bacteria, with still no growth. Could we be genuinely "missing" growth on his patients, or are we expected to "find" growth, just because his office UA showed "POSITIVE"? Out of thousands of physicians we serve, he is the only one with those concerns. I appreciate any feedback. Prafull C. Shah M. Sc.; M.S. Director of Microbiology Laboratory Doctors Laboratory, Inc. Valdosta, GA
(answered 06/30/2005)

mixed urogenital flora greater than 100,000 colony forming units. Do I treat this as a bladder infection
(answered 06/20/2005)

In clean catch urine cultures where the Urinalysis shows increased WBCs,Leuk Esterase,etc.,(or diagnosis UTI and no Urinalysis is ordered), -with vaginal contamination present e.g.50-100k lacto,etc., and Ecoli in small numbers,even below 10k,do you work up the Ecoli in these cases where it is is the only potential pathogen?
(answered 05/27/2005)

My lab is considering putting a default mechanism into our LIS that will cancel a urine culture ordered by a clinician if the urinalysis shows all of the following results: 1-clear urine 2-nitrite negative 3-leukocyte esterase negative We have heard of a few other labs that do this, does anyone out there have any experience with this procedure? How did it work out for your lab? Any information would be appreciated. Thanks!
(answered 03/02/2005)

We are looking to find some journal articles or documentation to aid us in setting uniform criteria for relexing an urine culture order from a routine urinalysis. Currently, many doctors are asking us to order an urine culture if it meets their own specific criteria from the urinalysis dipstick and microscopic exam. Their criteria vary greatly and it is very cumbersome to be sure that we are ordering cultures when they want them. Any information would be appreciated.
(answered 02/09/2005)

With regards to urine culture work-up: Would it be reasonable to routinely use the Chemstrip LN test to help determine when to perform susceptibilities on mixed cultures? For instance, an indwelling-cath specimen from a 70 y/o female that has a negative LN strip but grows out 50k each of 2 enteric gnrs, 10-50k Enterococcus, and <10k S.aureus. In this situation, NO susceptibilities would be performed. Thanks in advance for any thoughts on the matter. Glenn Farish MT(ASCP)SM DCH Regional Medical Center Tuscaloosa, AL
(answered 02/07/2005)

What should the colony count be to work up a pure culture on a voided urine?
(answered 01/17/2005)

How should I perform the count of CFU/ml in urine cultures under the kass method?
(answered 12/21/2004)

How important is it to run routine, clean-catch urine susceptibilities on patient from the Emergency room when they have been or will be treated with antibiotics?
(answered 11/24/2004)

How many days or hours should a cath urine be held? Our current procedure states that any invasive procedure be kept for 2days the technologist that wrote the procedure did not feel that a cath urine was considered invasive, she was considering only a urine obtained supra pubic to be an invasive procedure.
(answered 11/22/2004)

The Ped Team from ED at our institution is strongly suggesting that the lab should call a physician on the ped side of the ED any time there is a positive urine culture in children under 2 years. Are other institutions doing this? Thank you
(answered 11/22/2004)

Is S. saprophyticus a significant isolate in a male urine?
(answered 11/10/2004)

I attended the ASM early morning sessions by Barenfanger/Schwarzenberger on urine culture workup. They recommended doing a Bacticard strep for BE/PYR/LAP and if all positive identifying Enterococcus sp. They stated that all (100%) Enterococcus sp. are sensitive to ampicillin. That could be a canned comment and testing for Resistance to vancomycin for epidemiological purposes only. That is being met with resistance at our hospital. If we ID the Enterococcus by Bacticard ID and use an ampicillin and vancomycin kirby bauer sensitivity, should that be all the workup we need for urines?
(answered 11/09/2004)

Is there published national standards for urine culture contamination?
(answered 11/04/2004)

what is the clinical importance of streptococcus viridens isolation from urine?
(answered 10/28/2004)

What is clinical importance of Streptococcus pneumoniae findings in urogenital system?
(answered 09/26/2004)

Do you find it beneficial/cost effective to routinely set up a gram positive selective media such as PEA or BEA on all urines (along with the BAP and MAC) in order to catch significant isolates of Enterococcus from mixed cultures?
(answered 09/07/2004)

What is the clinical significance for the findings of S. epidermidis in >1000000/ml in urine in patients suffering from UTI?
(answered 08/05/2004)

I have got the information from a net based site that E coli is the most common source of UTI,which i am aware of but it mentioned that the next most common pathogen is Staphylococcus saprophiticus,is that true?
(answered 07/26/2004)

At what colony count is the cut off for identifing and performing sensitivties recommended?
(answered 05/03/2004)

To perform urine culture on pediatric patients admitted to ER/inhouse regarless of UA result. Is there literature to support this?
(answered 04/19/2004)

Is it standard techique to dip the calibrated loop just below the surface of the urine when doing a urine culutre?
(answered 04/12/2004)

We are currently updating our microbiology procedures for urine cultures (We are the lab for a large, 30 physician urology group) Has anyone utilized the BBL Chromagar orientation agar available from BD? In addition to the bi-plate, we are looking at using the Chromagar for isolating mixed cultures, particularly from catheterized urines,nephrostomy tubes,& suprapubic tubes. Also any ideas on QC requirements from this type of specialized media, or is the manufacturer's QC statement adequate? Hoping to gain some unbiased opinions (not from the manufacturer)
(answered 04/08/2004)

Is it ever necessary to use both a 0.01 and 0.001 calibrated loops to set up urine cultures? We use the 0.001 loop for clean catch specimens and add the 0.01 loop on BAP for cath specimens. Could the 0.01 loop be dropped from our protocol or would we be missing possible pathogens?
(answered 04/02/2004)

I want to know if in patients with chronic cystitis it has been shown that a reservoir of bacteria persists and when antimicrobial therapy is stopped the infection recurs? Also, after long term nitrofurantoin therapy do infections still recur? Is there any cure for chronic cystitis?
(answered 11/14/2003)

Do you have any recommendations or references on workup and reporting voided urine specimens. This is primarily the urines with colony counts 10,000-100,000. We report "Multiple different organisms isolated,probable contamination. Review clinical situation to determine if repeat culture is necessary";but we don't put a colony count on with this statement. Should we: 1)Enumerate, e.g. 30,000 multiple organisms isolated,etc... 2) say 10,000 cc viridans strep,10,000 lactobacillus and 10,000 coag.neg staph 3)30,000 cc, 3 colony types present. We want to generate the most helpful/useful report to the MD,but don't want to create a microbiology workup nightmare.
(answered 08/07/2003)

What would be considered an "acceptable" % of contaminated urine cultures for QA study?
(answered 04/27/2003)

HOW DO WE TRANSPORT URINE SAMPLES IF IT TAKES >THAN 20 HOURSTO REACH THE LAB?
(answered 04/01/2003)

What is the minimun amount of hours to hold a urine culture before the final report.
(answered 02/14/2003)

What is the current recommendation for working up multiple organisms in cathed patients in long term care situations?
(answered 07/22/2002)

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