Culture, wound

Culture, wound

DO YOU CONSIDER GROWTH IN THIO ONLY TO BE A CONTAMINANT? THERE IS NO GROWTH ON ANY PLATE EXCEPT OUT OF THIO. ONE SPECIMEN IS A CSF WITH GNR. AND ONE IS A WOUND WITH GPC.
(answered 04/29/2007)

DO YOU CONSIDER GROWTH IN THIO ONLY TO BE A CONTAMINANT? THERE IS NO GROWTH ON ANY PLATE EXCEPT OUT OF THIO. ONE SPECIMEN IS A CSF WITH GNR. AND ONE IS A WOUND WITH GPC.
(answered 04/29/2007)

We are writing new procedures for wound cultures. I have mixed feelings for setting up broths on all wounds. Many techs spent unnecessary time gram staining every thio. I want to keep the thio only for looking for anaeobes. Is this a good idea? Or should I eliminate the broth setup? Is chopped meat better? Do we have to boil the thio?
(answered 02/12/2007)

We have a physician that insists that he does not want enrichment broths setup on his surgical specimens. Is there somewhere that we can find documentation that enrichment broths are beneficial for sterile sites?
(answered 02/05/2007)

what critera is used to work up bacteria in a wound culture? We always work up Pseudomonas, Staph aureus, and mention beta streps. Are other gram negatives important?
(answered 01/30/2007)

Is there a easy way of determining the quality of a wound specimen using the gram stain results. We are currently writing a new procedure for wound cultures. we want to use the gram stain as a tool for determining what organisms to work up. Thank yo
(answered 01/30/2007)

Where might I locate a procudure for quantitative wound cultures for a hyperberic unit?
(answered 11/07/2006)

WE HAVE A PHYSICIAN THAT ROUTINELY SENDS ESCHAR TISSUE FROM NECROTIC ULCERS FOR CULTURE. IS THERE ANY CLINICAL SIGNIFICANCE TO THE ORGANISMS ISOLATED FROM THESE SPECIMENS AND HOW DO YOU HANDLE REQUESTS FOR SUCH SPECIMENS?
(answered 07/20/2006)

I have heard that quantitative wound culture is no longer offered by some institutions. Another DivC member put this out on the list server but got no responses. Has the procedure gone out of favor? If we haven't had a request in over 2 years ,would it be reasonable to drop it due to lack of expertise?
(answered 10/31/2005)

resulting clinical specimens like wounds how should they be reported (many few ...; +1,+2... or just the organism with no value??
(answered 10/31/2005)

Some of our orthopedic physicains are questioning the time frame in which we final wound cultures. After speaking with some of the other laboratories in our area, I am even more confused. Everyone handles the cultures differently. Do you have a general recommendation for when a negative wound can be finaled? What is the recommended time to hold plates and thio? Is is recommended to throw out the thio as soon as the culture is final? Same questions for negative anaerobic cultures.
(answered 10/26/2005)

How should wound specimens be worked up and reported if the direct gram smear indicates a poor quality specimen (i.e. no PMNs and an abundance of squamous epithelial cells or more SEC than PMNs) and there is a predominant amount of either S.aureus, Ps.aeruginosa, or beta hemolytic streptococcus in the culture?
(answered 10/25/2004)

We consider wound swab specimens to be of poor quality, as indicated in the 8th ed of the Manual of Clinical Microbiology, if SEC are equal to or greater than PMN in the direct gram smear. My questions are: a) is it appropriate to discard wound swabs that are considered to be poor quality and report only the direct gram smear result? b) should these types of specimens be cultured for potential pathogens (e.g. beta hemolytic streptococci, S.aureus, or P.aeruginosa)? c) what quantity of potential pathogen is considered significant before being reported from these types of specimens?
(answered 10/06/2004

dog bite pathogens/recurrent cellulitis Could you please tell me if pathologists can gram stain a skin biopsy for fungi, afb, dog pathogens, after it has been placed in formalin? If so, how accurate are the results? Thank you!
(answered 10/03/2005)

What significance should be placed on enterococci when found in wound cultures?
(answered 09/03/2004)

I would like to know how to work up a perirectal abscess.
(answered 08/01/2005)

I WOULD LIKE TO KNOW WHAT SHOULD BE CONSIDERATED A PATHOGEN ON A PERIRECTAL SWAB. THANKS
(answered 07/29/2005)

I would like to know the methodology used in collecting bacterial samples from diabetic foot ulcers using the needle aspiration technique
(answered 06/30/2004)

I have two questions that have to do with the work up of decubitus ulcers. After returning from an ASM sunrise session last year, I had our LIS person put in a question about surgical debridement as a prerequisite to ordering these cultures on the "order entry" screen. Most floors just answer "yes" so they can order these. What is the work up recommendation for these types of specimens? For example today we had a sacral decubitus specimen that grew out 3 different gnr's including an enterococcus. I advised the technologist to do a descriptive id only without susceptibilities. We hold our plates for 7 days. How do I respond to questions as to whether or not one of the gnr's is an "ESBL" since I've only provided a descriptive id?
(answered 06/11/2004)

May I have a reference for rejecting perirectal abscesses cultures?
(answered 05/10/2005)

Could you give us a reference for Aerobic Quantitative Tissue Cultures? We need a detailed procedure.
(answered 05/06/2004)

We often receive wound swabs that have organisms but no wbc in the direct gram smear. On culture, a pure or predominant growth of potential pathogen (i.e. S.aureus, coliform, or P.aeruginosa, etc.) may grow. How significant are these isolates in the absence of wbc and should susceptibility testing be performed?
(answered 10/17/2003)

Do you recommend gram stains on all wound cultures as a routine work up procedure? We do not perform an anaerobic culture on all of our wound cultures. We only do the anaerobic culture when appropriate and only do the gram stain when requested on these cultures. Do you recommend a gram stain as part of the routine workup on all wound cultures whether there is an anaerobic culture ordered or not? We do set up a thio on all wounds and check the thio for growth.
(answered 02/09/2003)

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