Mycobacteriology

Mycobacteriology

mycobacteria is it ok to heat fix afb stains at 80 degrees celcius for 15 mins?

(answered 05/11/2007)

does qc need to be perform on middlebrook 7h11(nonexempt) by the user lab? also which control organisms should be run?

(answered 05/07/2007)

mycobacterium other than tuberculosis was reported by pcr in endometrial cureettage, is it significantor contaminant/ which are the significant mott in endometrium ?

(answered 04/21/2007)

My hospital has asked me to answer a question regarding negative room pressure, and I can't locate an area for advise. Our Microbiology Dept. was a negative pressure room because AFB was performed in it as well as regular Microbiology and Mycology. We have gone to referencing out our AFB and need to know if a negative pressure room is still necessary for routine Micro/mycology. We have a hood that is sufficient and is routinely checked, but is there any CAP guidelines that require the need of a negative pressure room? Facilities claim that eliminating the negative pressure room can save the hospital money, and I would like to know where to retrieve information if it is necessary.
(answered 02/24/2007)

How are you performing QC on pH strips used in the concentration of specimens for AFB cultures for cystic fibrosis patients.
(answered 01/29/2007)

Is CO2 necessary to cultivate Mycobacterium spp. from clinical samples?
(answered 01/11/2007)

m.kansaii is most commonly isolated from which site?whichis the best system to isolate it and why?
(answered 11/13/2006)

In a level 2 laboratory, is it necessary to don disposable lab coat when processing AFB specimens under the hood? Can you provide refernce of this. Thank you.
(answered 11/07/2006)

Is it necessary to redigest AFB specimens if only the liquid medium shows contamination but the solid medium is not? If it isn't, can you tell me where the documentation can be found?
(answered 11/04/2006)

I had a wound swab of a post-op patient growing AFB in 48 hours on routine culture media.Patient was put on clarithromycin for 6 weeks.A repeat wound swab from another site shows similar organisms.What are the treatment options now ?We are refering the isolate for speciation and sensitivity testing.
(answered 11/04/2006)

which is the best website for information regarding the cultering of ntm?
(answered 10/18/2006)

Are AFB gram positive or gram negative? Is there a special way to perform the Gram stain when staining mycobacteria?Thank you
(answered 08/31/2006)

which is the commonest ntm causing submandibular abscess and its sensitivity pattern?
(answered 08/28/2006)

MY NAME IS SHUAIB UBA ABBA FROM ZAMFARA STATE, NIGERIA. HOW LONG DOES AFB REMAIN IN THE SPUTUM OF TB TRAETED PATIENT ? AND WHEN IS HIS SPUTUM WILL BE AFB NEGATIVE?
(answered 08/28/2006)

Why Mycobacterium in acid fast staining appear beaded in nature
(answered 06/29/2006)

What is the significance of doin TB IgG,IgM .There is no WHO STANDARDISTION for these kits.So how does one interpret a positive result in the absence of Positive signs & symptoms,or Mantoux test.Dr Pravin Nair
(answered 06/28/2006)

Our clinical lab will be moving to a new location on site, and management does not think we need an autoclave for positive AFB specimens. Does anyone have any references to support either autoclaving on site before outside incineration or is outside incineration only acceptable?
(answered 06/28/2006)

We are in the process of setting up the Cellestis Quantiferon-TB assay in our laboratory. The test materials as purchased from Cellestis does not include external positive and negative controls that are processed the same as the patients. Is this considered acceptable practice and are you aware of any copetency samples that are available? Thank You
(answered 06/05/2006)

BACTEC MGIT AFB validation
(answered 05/21/2006)

we routinely do an AFB stain on urines that have leucocytes, no antimicrobial agents, and no growth after 48 hours. We have picked up M. tuberculosis this way, but the yield is very low. Is there a leucocyte cut-off we can use, below which it is very unlikely to be TB, and therefor not necesarry to do ZN on the urine?
(answered 05/29/2006)

We grew rod shaped organism from a spinal wound culture (probably a decubidus) that turned out to be Kinyoun positive but Auramine negative. Could a Mycobacteria do this? or is this some other "bug"? The gram stain showed a beaded weakly gram positive rod. We tend to think it might be environmental. We do a lot of TB processing and smears in our lab and this looks like nothing we have seen in the usual TB type specimens (mostly respiratory). It had kind of a funky musty odor, grew well on BAP and Choc. after about 3-4 days. Was large (3-5 mm)creamy, dull surface, rough edges.
(answered 04/12/2006)

If the Acid Fast bacilli are dead when present in the sputum sample would it take up the Acid Fast stain when the sputum smear is stained by the Acid Fast stain? would greatly appreciate if you could provide me some refrence in this matter too.Thanx a lot for it.
(answered 04/19/2006)

temperature range for fixing acid fast smears
(answered 04/19/2006)

In the Guidelines for Preventing the Transmission of Tuberculosis in Health Care Settings it is mentioned that 3 specimens should be submitted for AFB smears and cultures 8-24 hours apart, with at least one being an early morning specimen. Our Infection Control Coordinators are requesting cultures 8 hours apart so they can get patients out of isolation quickly. Our lab policy is to accept sputum specimens 24 hours apart, preferably all first morning specimens. Should we adjust our policy to conform with the CDC guidelines for Infection Control measures? Do you have any references that provide information on the sensitivity of this process?
(answered 04/19/2006)

Based on previously reported in the literature,to rule out TB, one needs three negative AFB sputum smears collected on three consecutive days. Has this practice been updated by CDC or IDSA to three negative sputum AFB smears collected q 8 hours?
(answered 02/01/2006)

May we use distilled water as buffer in the NALC decontamination method?
(answered 01/23/2006)

What would you recommend we do when we receive a wound specimen on a swab for AFB? Some of my techs swab it onto 2 LJ slants(30 and 37 degrees)and then make a smear while others place the swab in a small amount of saline and then use the saline to set up 2 slants, a smear, and a myco/F sputa bottle. We realize that a swab is not the specimen of choice but our pathologists do not want us to reject the specimen.
(answered 10/27/2005)

Are there any regulations regarding if a acid fast sputum smear is positive. How long before a second sample should be sent.Should the first positive be confirmed by sending another sample?
(answered 10/26/2005)

For acid fast smears if an initial smear is positive and a physician waits two weeks to send a second sample. If the second sample is postive again Should the whole process of getting three consecutive negatives start all over again? Or is that 1 second positive enough?
(answered 10/26/2005)

Does anyone perform Quantiferon-TB testing? How expensive is this procedure? How labor intensive is this procedure? Thank you.
(answered 10/13/2005)

IS IT NECESSARY TO HAVE A REFRIGERATED CENTRIGUFE WITH SWINGING BUCKETS TO PROCESS MYCOBACTERIUM SPECIMENS?
(answered 10/13/2005)

How do you pronounce "mageritense?"
(answered 10/07/2005)

Does MYCOBACTERIUM grow on blood agar media? In our laboratory we recognize growth of acid-fast bacteria on blood agar when turbid liquid media (7H9) sub cultured ?? THANKS
(answered 09/08/2005)

I have been asked whether or not a TB skin test (ppd) should be read and interpreted by the technologists in Microbiology. I was trained that as a MT that these types of test s were performed and interpreted by a clinician. What is the take of the panel and are there any references?
(answered 07/06/2005)

In our mycobacterial and legionella laboratory we use auramine stain as primary stain for detection of acid fast bacilli then for conformation followed by ZN. After treatment started is there any stain can be use to detecte the viable and non viable bacilli?please if available give use the full details thanks yours,truly bilal
(answered 07/06/2005)

Migit Media QC what are the requirements for QC on migit media if you read the reaction manually instead of using an automated system.
(answered 06/23/2005)

after making smear for AFB(AURAMINE OR ZN STAIN),is it necessary to air dry only or heat dry ?
(answered 06/20/2005)

Is it appropriate to perform smear and culture or just culture on stool specimens for mycobacteria? Any references?
(answered 05/18/2005)

If a fluorescence stained sputum smear is positive for AFB, doyou still recommend de- and re-staining by ZN to confirm the +
(answered 05/04/2005)

In case of a fully sensitive M.tuberculosis and correct treatment is there a rule of thumb for what is the expected time of negativation of the smears? Will cultures be positive for a shorter or longer time? In other words, how long after initiation of therapy does it take for culture and AF smear to become negative?
(answered 10/28/2004)

Due to the low numbers of AFB requests that we receive for blood we cannot use the Isolator system (expiration issues). For blood we inoculate a heparin tube and then do a 5 minute NALC digestion, add buffer and then concentrate. Do you feel that this is adequate? With bone marrow specimens, we do direct inoculation to media plates and a 7H9 broth (MGIT is used here and so we inoculate 7H9 on any bloody specimen instead). But, I was wondering if we should be doing a lysis step for these specimens also. If so, could we do the same process as for blood (put it in a heparin tube, etc.)?
(answered 10/15/2004)

Do you have a suggestion for doing AFB smears on pleural fluids? Even if the smears appear thin visually, whenever they are stained with the fluorescent stain the entire inoculated area looks thick and "knock your eyes out" bright. We question whether we would be able to see AFB if they were present. We've tried to dilute the specimen with some saline, but that doesn't help much - and defeats the purpose of a concentrated smear. Our guess is that it's the protein that is causing the problem.
(answered 10/15/2004)

Can a false positive on a TB skin test be brought on by taking tests weeks apart or what other things can cause a false positive in a healthy patient?
(answered 08/31/2004)

what other bacteria or viruses produce a positive tb skin test?
(answered 08/04/2004)

Does mycobacterium smegmatis form edospores?
(answered 07/09/2004)

Is there a required time and temperature for heat fixing smears for acid fast staining( auramine M stain)?
(answered 07/01/2004)

Is mycobacterium bovis really different from M. tuberculosis?
(answered 06/30/2004)

Are there any regulations concerning how long TB cultures must be held?
(answered 06/08/2004)

AFB- sputums that are smear or culture positive for AFB-if there are multiple specimens collected are there written protocals that describe that each positive specimen is probed for mtb, or is ok to only probe the first specimen. Is there a reference
(answered 06/07/2004)

Is the determination of adenosine deaminase for the diagnosis of pleural tuberculosis a specific and sensitive test? Where can I find information about it? What are normal values? Is it useful for CSF?
(answered 04/20/2004)

how can we diagnose lung tuberculosis by manto test?
(answered 04/12/2004)

What is considered a good rate of contaminated cultures for mycobacteria?
(answered 02/05/2004)

If you are inoculating a specimen for AFB immediately after finishing the digetion and decontamination, is it necessary to use bovine albumin? Does the bovine albumin do anything other than helping the inoculum adher to the media and if you are using broth media (such as the BacT/Alert) is it necessary?
(answered 08/05/2003)

Does Mycobacteria stain with Giemsa stain? And if so, what color would it be?
(answered 07/16/2002)

Would you clarify the BSL required by a diagnostic lab regarding TB cultures
(answered 04/01/2003)

What are the current safety standards that Mycobacteriology Labs must meet to be in compliance?
(answered 02/21/2003)

Is Mycobacterium simiae a true pathogen when it is cultured from BAL?
(answered 02/20/2003)

Does the panel have any experience with in vitro susceptibilities for gatifloxacin and moxfloxacin against M. chelonae and M. abcessus
(answered 02/14/2003)

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