Other

Other

Can you explain why the CLSI removed the disc zone interpretations for Pseudomonas aeruginosa and trimethoprim/sulfamethoxazole in 2006? We only report TMP/SXT Kirby Bauer results for P. aeruginosa on our cystic fibrosis patients. The physicians still want us to report the TMP/SXT results. Is it ok to report the "old" interpretations or is there an issue with the disc results vs. MIC results? Thanks, in advance!
(answered 06/20/2007)

How significant is it to identify and setup sensitivities on CNS from sterile body sites? Such as knee or joint fluids.
(answered 06/20/2007)

Are there any guidelines for what antibiotics should be reported on a Shigella from an extraintestinal source?
(answered 05/31/2007)

QC Susceptibility Testing on a New Antibiotic. We routinely use MicroScan panels for our susceptibility testing. I am being asked by one of our physicians to include a new "study" antibiotic on selected respiratory isolates. This new antibiotic is not on any Microscan panel and will appear on the patient's medical record. The study protocol suggests using E-test for this new antibiotic. What would be the appropriate QC protocol for such a situation? I was thinking of choosing the one (of five) QC organism that is closeset in identification to the patient isolate and running one of the five QC organisms each day of use. I suppose if I had two patient isolates, a Staph and a Pneumo to test on a given day, I would have to run both the Staph and Pneumo QC organisms on that day because of the different MH media requirements. Does this plan sound right?
(answered 05/21/2007)

Can interpertive guidelines for staphylococcus aureus be used for staphylocccus lugdenensis if an automated system is used to determine susceotiblities, i.e., key-id staphylococcus aureus for staphylococcus aureus?
(answered 05/21/2007)

Is it acceptable to use Mueller Hinton Sheep Blood for susceptibility testing on the day it expires?
(answered 05/21/2007)

Hello: Is there a procedure or protocol to determine if the spread of VRE in a particular area or unit is the same organism or just randomly isolated separate organisms? Where or how can these isolates by typed to determine a possible source? Thank you. Carol Wegman MT(ASCP)SM Microbiology Technical Specialist St.Joseph Medical Canter Reading PA 19605 phone 610-208-8845 fax 610-208-8846
(answered 05/15/2007)

If antimicrobial discs are left out at room temperature overnight, will their sensitivity to organisms decrease?
(answered 05/10/2007)

Gram Neg. Rod's Multiple Drug resistance, which patterns should be reported for isolation in a hospital? And for Strep. pneumonieae wich is considered MDR pattern to report? Thank you

(answered 05/10/2007)

i want to purchase clini cal manual of microbiology but i,m confused whether to buy clinical manual o fmicro or prcedures handbook in microbiology from asm. first u tell me what is the differance between the two? will manual in clinical microbiology cover everything given in handbook proceduers in clinical microbiology?pl. answer this urgently.
(answered 05/08/2007)

SHOULD NALIDIXIC ACID BE RUN ON URINE CULTURE KIRBY BAUER PANELS FOR E. COLI?
(answered 05/08/2007)

Our microbiology department make our agar plates in house. To preserve the quality of the plates they package them in plastic bags and seal them to prevent drying out. They also put packets of desiccants in the bag. This seem to be a bit of a paradox. Could someone enlighten me because it seems thst the desiccants should not be in there.
(answered 05/07/2007)

IN THE NEW CLSI STANDARDS THERE ARE INTERPRETIVE CRITERIA FOR DAPTOMYCIN ON STAPH BUT NOTHING IS SPECIFIED FOR ENTEROCOCCUS-- CAN DAPTOMYCIN BE TESTED ON BOTH ENTEROCOCCUS FAECIUM ( VANCOMYCIN RESISTANT) AND OR ENTEROCOCCUS FAECALIS ( VANCOMYCIN SENSITIVE)
(answered 05/02/2007)

are c.diff.specimens suppose to be refrigerated?

(answered 04/27/2007)

Are there any published zone sizes for Shigella sonnei isolates to azithromycin? Azithromycin has been indicted for the treatment of Shigella sonnei, but I cannot find testing requirements.
(answered 04/25/2007)

I need to find the breakpoint MIC's for Colistin/Pseudo aeruginosa.
(answered 04/25/2007)

What is the difference between yeast and yeast-like ?
(answered 04/24/2007)

why are pure cultures used for antimicrobial susceptibility testing?
(answered 04/22/2007)

why does neisseria gives positive result for catalase tests
(answered 03/20/2007)

bench proceduresour laboratoryis trying to update all of our procedures,urine,wound respiratory,genital,stool,and screening as we are without a supervisor or senior tech.we are at a loss as how to get the best results.this is a huge task.all comments and hints are most welcome.
(answered 03/18/2007)

Is it appropriate to include a therapy related comment from a reference microbiology text on the patient report for organisms that have no standardized susceptibility method available or routine susceptibility testing is not necessary, e.g. Pasteurella from bite wound?
(answered 01/11/2007)

what does the result of a positive 2+ gram positive staph aureus indicate?
(answered 12/11/2006)

Will the new CLSI susceptibilily standard (1/07) include breakpoints for tigecycline? We have been using the E-test and reporting FDA breakpoints, but there are no FDA breakpoints for testing acinetobacter baumanii strains with Tigecycline. Thanks, in advance.
(answered 12/05/2006)

WHAT CAUSES STREP VIRIDENS IN URINE CAN YOUR UA BE NEG AND STILL HAVE STREP VIRIDEN IN YOUR URINE?
(answered 11/20/2006)

Which antibiotis are appropriate to prescribe after a blood culture is found to have gram positive bacteria?
(answered 11/20/2006)

We have almost completed our 20-day QC for our new Vitek 2 instrument. The weekend tech failed to set up one day, and now we have a gap in our data. Can we continue from this point without penalty, or must we start the 20 days all over again?
(answered 11/13/2006)

FOR GRAM NEGATIVE RODS CAN IMIPENEM AND MEREPENEM SUSCEPTIBILITY BE USED TO PREDICT SUSCEPTIBILITY TO ERTAPENEM???
(answered 11/07/2006)

I would like some turn-around-time guidelines for Microbiology critical values. Where is a good place to access them?
(answered 11/03/2006)

To assess tobramycin sensetivity for Pseudomonas should MIC or Kirby Bauer be used?
(answered 10/27/2006)

Our laboratory centrifuges CSF and other body fluids for 15 minutes at 3200 rpms. Is there a better way to process these specimens? Are we centrifuging too long and too fast? Please give me some advice on how to process these specimens better?
(answered 10/27/2006)

how to perform schlichter test correctly?
(answered 10/26/2006)

is gentamycin testing enough in esbl +ve case to predict amikacin results also?
(answered 10/12/2006)

if your not pregnant and get beta strep group b how is that possible? and what could be causing its over growth? could it be semen?
(answered 10/11/2006)

Do all Cultures need to be incubated in Co2?
(answered 10/11/2006)

i had a e.coli esbl+ve isolate from urine culture showing resistance to nalidixic acid, ofloxacin but sensitive to getafloxacin and levofloxacin so how should i report ast w.r.t quinolones in this case? is there any protocol for quinolones in particular?
(answered 10/09/2006)

in a esbl +ve strain is it necessary to test imipenem and meropenem separately? if so then why?
(answered 10/09/2006)

in my enterococcus feacalis is showing sensitivity to all cephalosporins so should i report it sensitive to all?
(answered 09/05/2006)

which are the antibiotics disc to choose and which to reject while testing for enterobacter and why?
(answered 09/01/2006)

We have isolated Gram negative diplococci from a urethral discharge,which grows luxuriantly on Thyer Martin agar,it is oxidase positive,weak catalse positve and ferments glucose and maltose and the vitek 1 has identified as Neisseria mengitidis,i know it is an obvious case of Neisseria gonorrhoea but then why am i having these results could you please guide me and would very much appreciate a prompt reply,thanks.
(answered 08/18/2006)

how to proceed in UTI when organism is NLF, gram negative bacili, citrate and urease negative, on TSI acid butt and acid slant with gas. can it be a e. coli?
(answered 08/10/2006)

if the zone of inhibiton around strep pneumo is 20 and the acceptable limit is 20, is this isolated sens or res?
(answered 07/26/2006)

test questi9ons jmdcojicjaso
(answered 07/25/2006)

Antifungal susceptibility testing of Yeasts by E-test method- is it recommended for clinical use, by CLSI?
(answered 07/24/2006)

Is EC35218 required to be performed for QC on Pip/Tazo KB disks? We are only testing Pseudomonas patient isolates.
(answered 07/11/2006)

why blood agar was used in addition to Mueller-Hinton agar for the disk diffusion technique?
(answered 07/11/2006)

We are doing 30 day QC for Cefepime disks so that we can run it as a double check for P. aeruginosa coming from Microscan. We will do QC with PA27853, but are we required to run it with EC35922 as well?
(answered 07/11/2006)

we can use van soest medium for culturing of aerobes or not? if yes how? if no then which medium can be used?
(answered 06/15/2006)

How much of a difference do you find between the microdilution method and the macrodilution method? We are testing drugs not typical antimicrobials and have found some differences between both methods.
(answered 06/06/2006)

Can someone give me a resource to find the proper procedure for the D zone test to check clindamycin resistance in Staph species?
(answered 06/06/2006)

Is there any valid reference permitting to report "intermediate" interpretation results of routine in vitro antimicrobial sensitivity results as "resistant?"
(answered 05/02/2006)

Can I (a hospital lab) charge for a confirmatory identification that was sent to the State health Laboratory (i.e. a Mycobacterium isolate sent for speciation by probe or HPLC)?
(answered 04/04/2006)

The CDC/NIOSH folks and the WHO are recommending use of N95 masks by doctors during an avian flu pandemic. These masks are 95% affective at trapping particles which are .3 microns or larger. Virus particles are 1/10 that size (about .02 or .03 microns). How could these masks protect our first responders? The CDC was not able to figure that Anthrax spores are smaller than the pores of a paper envelope and thus save postal workers lives. Is this just more of the same type of ineptitude?
(answered 03/31/2006)

When developing an antibiogram NCCLS recommends including only the first species specific isolate per patient during the time period. What is the best way to handle a patient with a primary culture yielding mutliple biotypes, of for example E.coli, with different resistance patterns?
(answered 03/04/2005)

What QC organisms are used for cefinase beta lactamase testing?
(answered 03/29/2006)

Our lab uses the Wampole LA Cryptococcal Ag kit. We have issues with some sera clotting in the heated deactivation phase rendering the specimen unusable. I have been told by the manufacturer that this phenomenon is interferance and cannot be prevented. Is anyone familiar with this problem and how does your lab combat the issue ?
(answered 03/29/2006)

List of organisms with their natural resistance patterns
(answered 03/29/2006)

Stenotrophomonas maltophilia,Antibiogram Q. Can Stenotrophomonas maltophilia be susceptible to majority of antibiotics including Ampicillin? Is there any nonfermenter which is susceptible to Ampicillin?
(answered 03/29/2006)

1.can the zone of inhibition be used for the potency/ strenght of an antibiotics 2. I have double zone of inhibition for cloxacillin/ampicillin assay using staph.aureus in the same plate. Is it possible and how do I determine each active strenght. 3.in a mixture of both active ingredient how do one determine each active using the zone of inhibition measuremet. t
(answered 03/09/2006)

Is there a table or reference anywhere which discusses inherent resistance among specific organisms? Using automated systems is wonderful, however, they are not absolute. And sometimes, although expert rule systems will be flagged, repeat testing may yield identical results. Being a small lab which ordinarily would not refer such organisms, I cannot find a solid reference which lists inherent resistance patterns of specific organisms. Can you help?
(answered 03/09/2006)

Are there kits available for antimicrobial susceptibility testing that come with disks pre-empregnated with a broad spectrum of drugs?
(answered 03/01/2006)

NEW VANCOMYCIN INTERPRETIVE STANDARD FOR S.AUREUS.IS EVERYBODY FOLLOWING CLSI OR WAITING FOR A YEAR?
(answered 02/15/2006)

I am looking for a reference regarding the D zone test for Staph. species. Any suggestions?
(answered 02/13/2006)

In response to the question about techs wearing gloves when reading plates on the open bench, is it possible for ASM to provide me with the informal ASM listserve survey results concerning current practices mentioned in your response? Any information would be greatly appreciated.
(answered 02/07/2006)

treatment for Corynebacterium minutissimum - systemic infection
(answered 01/30/2006)

Is there a recommended treatment or protocol for Listeria and Erysipelothrix infections? Thanks
(answered 01/30/2006)

What exactly does the CLSI do with regard to creating documentation that the UK is supposed to follow for the antibiotic disc diffusion testing of bacteria? I believe the CLSI creats control and guidline documentation- but what exactly does this entail? media, dilution.....
(answered 01/24/2006)

Can you tell me whether or not a clinical study has to use a CLIA certified laboratory to perform their testing?
(answered 01/19/2006)

I teach medical technology students how to read gram stains using previous patient gram stains. I've tried to save some really good slides using permanent mounting media but I have noticed that gram positive organisms have been fading considerably and I end up having to remake slides. Is there a set procedure on permanently saving gram stains? What might I be doing wrong?
(answered 01/13/2006)

We are in the process of creating an antibiogram for our facility. I have generated a percent susceptible report from our analyser and noticed that for Nitrofurantoin we have 15% susceptibility for Enterococcus faecium and 96% susceptibility for E. faecalis. Different articles that I have read say that Nitrofurantoin is a drug of choice for Enterococci so why is my susceptiblity percentage so low for E. faecium?
(answered 01/13/2006)

Can oxacillin or vancomycin screen agar plates be used more than 1 time
(answered 12/22/2005)

What are your current rcommendations for GNR susceptibility testing methods? Any reference or written quideline you can provide will be appreciated.
(answered 12/12/2005)

we use an oxacillin disk to screen for penicillin susceptibilities in Strep Pneumo. Do we need to run a positive control as well as the negative 49619 Strep pneumo control?
(answered 12/07/2005)

I found the answer regarding group B Streptococcus and the D-test. What is the procedure for a viridans streptococcus D-test? i.e. Zone distance, agar type,and length and conditions of incubation?
(answered 12/06/2005)

I have observed that when i put cepholosporins eg.caftazidime and cefepime about 20mm from imipenam with pseudomonas aeruginosa around the cephalosporins bordering imipenam there is bluntuing of the curved zone(like the D effect around clindamycin when kept near erythromycin-when there is induction),what could be this phenomenan? and also though the zones around all the 3 antibiotics are in the size to classify them as sensitive,should they be said so?hoping for a reply soon,thanks.ali
(answered 11/29/2005)

what are the symptoms or circumstances pneuminia
(answered 11/29/2005)

Is there any > established acceptable procedure for transferring blood from a sterile > vacutainer tube into a blood culture bottle? My situation has to do with > an ER physician wanting to transfer blood from an "extra tube collected > at initial venipuncture" to a blood culture tube. He states this as a > standard procedure from another facility. Our ER nurses often collect > blood for lab procedures including blood cultures, and they have been > instructed in the proper collection techniques. The issue arises when a > blood culture is not ordered at the initial collection and the ER > physician wanting to use that blood for the 1st blood culture. The cost > of blood culture bottles prohibits simply collecting them as extra > blood. So I guess my question is, if sterile technique has been used, > could you transfer blood from a green top or purple top and use it to > inoculate a blood culture? Thank you for any assistance you can provide > in this issue.
(answered 11/29/2005)

I apologize for having to ask this question again, but I could not retrieve the previously asked question about transport of sterile body fluids in plastic red top tubes versus glass red top tubes. Has there been any correlation with decreased recovery of isolates using plastic versus glass?
(answered 11/23/2005)

There was a brief article in the New Orleans Times-Picayune Newspaper titled "Fit to be Tied". Can you provide the research for neckties containing bacteria? (answered 06/02/2004)
(answered 11/17/2005)

Microscan has received FDA approval for new formulations for reporting vancomycin resistant S. aureus. Can we stop using the BHI w/vancomycin plates for confirmation?
(answered 11/13/2005)

cumitech
(answered 11/13/2005)

Are there controls for Cefoxitin screen for MecA mediated resistance??
(answered 11/09/2005)

IS TIGECYCLINE FDA APPROVED FOR TESTING AND REPORTING?
(answered 11/04/2005)

What is reproted when a Proteus species is resistant to ciprofloxacin and susceptibile to levoflxacin?
(answered 10/25/2005)

how much education is required for microbiologists
(answered 10/05/2005)

Is there a section on Forensic Microbiology within ASM
(answered 09/28/2005)

is cefoperazone-sulbactam fda-approved?
(answered 09/08/2005)

What is the bacterial and endospore cell size of bacillus thuringiensis?
(answered 09/02/2005)

What are the new, 2005, vancomycin mic breakpoints for MRSA and staph aureus?
(answered 08/29/2005)

We have been getting requests for Polymyxin B and Colistin for susceptibility testing. What is the best method and why are there no interpretations to date?
(answered 08/25/2005)

D test Procedure
(answered 08/21/2005)

Why is P. vulgaris H2S + and E. aerogenes H2S -
(answered 07/20/2005)

Stability,hazards etc profile of genetically modified bacillus mesentericus
(answered 07/11/2005)

journals+facial antimicrobial agents+ S. aureus & S. epidimidis Are there current journals and researches about the effects of antimicrobial agent to the antibiogram of both S. aureus and S. epidimidis
(answered 06/03/2005)

I am currently updating our sensitivity procedures and would like to know if intrinsic resistance is equivalent to expected resistance (the Manual of Clinical Microbiology has a table with expected resistance of organisms)?
(answered 05/08/2005)

Is it appropriate to change the interpretation (leaving the MIC unchanged) obtained with the Vitek system for Staphylococcus/Levofloxacin to reflect the tentative range and interp changes in the CLSI 2005 document. I have been told by a Vitek tech service person that labs are under no obligation to follow tentative recommendations, but I'm not comfortable reporting an isolate as "S" or "I" that would be considered "I" or "R" using the new range.
(answered 05/08/2005)

Do you know a procedure or reference to perform the induction d test on staph?
(answered 04/28/2005)

Hello, We recently had a problem peforming weekly susceptiblity qc on our MIC panel for Heamophilus. The tray containing the plates was dropped and of course the plates were contaiminated. Murphey's law being what it is, we were not able to get the necessary isolate ordered and growing until after the week had gone by! How should we handle this stitutaion? The MIC panel has not had any failures in the past and when we finaly got the ATCC organsim needed, the MIC panel passed QC. We did report patient results during this period. Should we go back and repeat the patients?
(answered 04/17/2005)

Could you email me the procedure for vancomycin screen for staph aureus as previously asked in 3/2005?
(answered 04/17/2005)

IS IT UNUSUAL TO HAVE STREPTOCOCCUS PNEUMONIAE THAT IS PAN SENSITIVE EXCEPT FOR THE FLUOROQUINOLONES? WE HAVE HAD 2 ISOLATES OF PNEUMOCOCCUS IN THE LAST WEEK THAT ARE RESISTANT TO LEVOFLOXACIN AND GATIFLOXACIN BUT SUSCEPTIBLE TO PEN, TRIMSULF, ERYTHO, CLINDA, TET, VANC, MEROPEN AND CEFTRIAXONE.
(answered 04/14/2005)

Can the polymyxin B breakpoints in the CLSI M7 2B table be used if colistin is tested by Etest?
(answered 03/02/2005)

Where can I find information on the latest emerging infectious diseases?
(answered 02/24/2005)

is cefoperazone and sulbactum effective in the treatment of esbls
(answered 02/15/2005)

describe the mechanism of inducing clindamycin resistance
(answered 01/25/2005)

D-test for beta strep- is it acceptable to use the purity check method (BAP) or is a Mueller Hinton sheep blood required?
(answered 01/25/2005)

how long can you have staph aureus before you have systoms
(answered 01/21/2005)

We currently test our GBS isolates on penicillin allergic obstetric patients against clindamycin, erythromycin and vancomycin per CDC guidelines. Numerous references state that macrolide inducible resistance can occur with streptococci. Should the D-test be performed with these isolates and if the testing is applicable should the same comment be used that NCCLS suggests for staphylococci that are D-test positive?
(answered 01/07/2005)

To determine colistin susceptibility of multi-resistant Acinetobacter calcoaceticus-baumanii complex, we intend to make Mueller Hinton agar plates containing 4 mcg/mL colistin. Is it necessary to adjust the weight of the colistin sodium methanoesulfonate to account for the 13,050 Units/mg listed by the manufacturer (SIGMA item C1511). Is colistin dissolved in water? Which QC organisms do you recommend for this single-level MIC?
(answered 01/05/2005)

we are trying to establish a realistic percentage of our mrsa,vre and esbl by counting patient numbers not isolates. are the published percentages based on isolate numbers or patient numbers
(answered 01/05/2005)

Where in the Internet could I find clinical microbiology discussion groups/chat rooms?
(answered 12/25/2004)

can you m.r.s.a cause liver infection
(answered 12/22/2004)

what are break points?
(answered 12/22/2004)

In setting up the Dtest, I was wondering what QC is recommended, other than just qcing the actual disks for 30days. would it be ok, to use an in house known positive staph for the d test, and the Staph #25923 for the negative? saw articles where different Cdc strains were used, how do we obtain these strains?
(answered 12/17/2004)

I've been looking for trends present in antimicrobial resistance. To do this, I need individual state data. I have contacted numerous health departments, the NCHS, NIAID, CDC, NIHC, APUA, ROAR, and multiple databases and I have been unable to find the data I need. Is there a place that is open to the public that has this data?
(answered 11/24/2004)

How many bacteriologist are there in the United States?
(answered 11/09/2004)

I am a medical technologist. I am working in microbiology in a hospital lab. I am interested in antibiotics (sensitive zone diameters)
(answered 11/09/2004)

susceptibility testing of penicillin and other beta-lactams for strep.pyogenes or strep.agalactiae is not necessary(nccls-m100s14).what are the current indication for testing and reporting of penicillin for beta-haemolytic streptococci gr.c or g.
(answered 10/20/2004)

I have a patient with chronic gonococci + ve treated with penicillin but no response. should both partners treated with ceftrioxin.
(answered 10/01/2004)

Can someone please inform me of correct procedure of how to set up quantitative cultures on BAL and PSB.
(answered 09/21/2004)

I am inquiring if there is a procedure for the D-test for Clindamycin when testing Staphylococcus species for inducible resistance to Clindamycin.
(answered 09/20/2004)

Could you please outline for me some similarities and differences in the care of a patient diagnosed with epidemic mrsa in an acute care teaching hospital compared with high dependency residential aged care (nursing home).
(answered 09/08/2004)

An aspirated sputum Gram stain had 10-25 WBC per LPF and 2+ Gram positive cocci in clusters. The culture had moderate growth of MRSA and scant growth of 2 kinds of Gram negative rods. No normal respiratory tract flora was present. What is the significance of the gram negative bacilli and how should these culture results be reported?
(answered 09/02/2004)

I have yet to receive a reply to my query of getting some more information about "occult hepatitis B".would very much appreciate any more information on this topic and if you could suggest me any related website regarding it.
(answered 08/04/2004)

Could you provide the "new" QC requirements for "routine" reagent testing to meet the revised CLIA standards (maybe in chart or column form)? The only references that I can locate were from July 18, 1997 ("Survey to Clinical Microbiologists on QC").
(answered 07/26/2004)

What are CLIA requirements for QC of Streptex reagents?
(answered 07/26/2004)

Vitek recently sent a memo regarding VRSA and suggested that an alternate method be used when reporting vancomycin results. Do you have a detailed procedure for setting up the vanomycin agar plate. Do I need to make a 0.5 McFarland dilution before setting organism on the vanc agar?
(answered 07/26/2004)

How many E. Coli organisms are needed to cause serious infection?
(answered 07/19/2004)

Is bacillus subtilis an acid fast bug?
(answered 07/08/2004)

We do not perform disc diffusion susceptibilities, but have started to set up D-tests on the purity plates for our Microscan automated system. We place the discs 15mm apart on the blood agar purity plates, streaked with the same inoculum that goes onto the panels. We have found a number of isolates with inducible clindamycin resistance. To validate this method, we send the same isolate to our reference lab, where the D-test is done as part of their disc diffusion testing. Two isolates that we interpreted as having the inducible resistance gene were reported as "negative" for the inducible gene at the reference lab. As far as we can tell, the only difference in our methods is that the reference lab places their discs 26mm apart, as suggested in NCCLS (15-26 mm for disc diffusion, 15mm for MIC's). Can you suggest a reason our results have differed? Why are the distances different for disc diffusion? Thank you.
(answered 06/02/2004)

There was a brief article in the New Orleans Times-Picayune Newspaper titled "Fit to be Tied". Can you provide the research for neckties containing bacteria?
(answered 06/02/2004)

what are labs doing to decrease rate of contamination in the MGIT?
(answered 06/02/2004)

What is the recommended guidlelines for QC testing of Vitek identification cards?
(answered 05/27/2004)

What is the recomended procedure that can be used to screen for GBS in pregnant women?
(answered 05/22/2004)

Is it possible to know that Cefoperazone+Sulbactam, a product used for therapeutic purposes to combat Gram Negative Septicaemia / Bacteremia & wound infections? Is this combination is on the approved list of FDA?
(answered 05/14/2004)

I am looking for voluntary research opportunity in Microbiology. How can I find one?
(answered 05/06/2004)

what organism produces an apple-green pigment on tryptic soy agar
(answered 05/06/2004)

How much ATP is produced from one molocule of butter fat containing one glycerol and three 12-carbon fatty acids
(answered 04/07/2004)

is antibiotic resistance a problem (locally or worldwide)?
(answered 03/23/2004)

my husband and i have just been diagnosed with mrsa, we have a 3 yr old and a 6 month old should we be concerned with them already having it, is their any way to test them for it if they dont have any wounds, how can we prevent them from getting it if they dont have it, will we always have this or after treatment will it go away, i just want to know anything about this you do. we were pretty shocked to find out we had it. and are confused how we got it so please help us in any way you can
(answered 03/21/2004)

I am looking for a list of the broad spectrum antibiotics.
(answered 03/21/2004)

In most of the bacterial diseases rifampin is used as profylaxis. Why not any other antibiotics?
(answered 03/21/2004)

I am looking for a lab that performs the following tests: Legionella IgG & IgM, Bartonella IgG & IgM, Francisella IgG & IgM, Q fever IgG & IgM, Coccidiodomycosis IgG & IgM, and Chlamydia IgG & IgM.
(answered 02/04/2004)

We would like to verify staff aseptic technique by having them inoculate 10 culture bottles with a sterile saline solution. Someone told us that saline should not be used because it inhibits bacterial growth in the culture media (we are using an aerobic culture). Does normal saline adversely affect growth in culture? Is there a standard process for staff evaluation in aseptic technique or is our proposed method acceptable?
(answered 11/12/2003)

I want to know the specific bacterial causing agents in cholangitis versus cholecystitis.
(answered 11/12/2003)

I am currently In Bio 205 (basic Micro) and have been given an assignment to find different controls used in Microbiological experiments ( at a college level). I know of Positive and Negative controls but any other commonly used controls you could help me with would be greatly appreciated.
(answered 11/10/2003)

What is the common microorganisms that can be found in the textile effluent and what is the procedures to isolate microorganisms from textile effluent?
(answered 07/30/2003)

If a culture is performed on all negative rapid group A strep antigen tets, can you bill for both the Rpaid and the culture?
(answered 07/13/2003)

Hi I am doing a health history paper for nursing school on a patient who aquired viridins streptococcus bacteria which resulted in endocarditis and a heart valve transplant this person states that he did not have any dental procedures or adhesions to the mouth prior to illness all that he can remember happening to him is 1 1/2 - 2 weeks before he had any symptoms he cut his right hand on a board at his construction job. I am needing to find out whether or not a open wound on the skin could be an avenue for this type of strep. bacteria to finish my paper
(answered 07/10/2003)

I have an Infectious Disease physician that insists that we not use the phrase "mixed skin flora" on wound cultures. Are there any references that I could provide to him to show him that especially on swab samples of wounds, when the gram stain shows no polys that this wound may be colonized not infected. Also are there any references or studies that show a swab sample generally is not the specimen of choice, but that a tissue biopsy may yield better results.
(answered 07/10/2003)

What is bad about Antrax?
(answered 07/07/2003)

What is bad about Botulism?
(answered 07/07/2003)

What is VHF and is it bad?
(answered 07/07/2003)

What is bad about the Plaque?
(answered 07/07/2003)

what is Tularemia?
(answered 07/07/2003)

How can I contact the web site, before 11th July 2003 (for reduced rate), to pay to attend 43rd ICAAC Chicago, 14-17 Sept 2003; the web site is unobtainable.
(answered 07/07/2003)

Ralstonia paucula
(answered 07/02/2003)

We occasionally see erythromycin resistant, clindamicin susceptible pattern in oxacillin susceptible S.aureus. Should we be concerned with inducible clindamicin resistance? Or does that only apply to oxacillin resistant staphs?
(answered 06/13/2003)

What QC procedures should I put in place during isolation of Salmonella from foods?
(answered 04/06/2003)

Where can I find a copy of Linda Nims report on the blind submission of avirulent B. anthracis to clinical labs in New Mexico- present at 101st General meeting 2001?
(answered 03/07/2003)

Recently we had a case of chickenpox and the lady is scheduled for caesarian section. The Obstetrician requests for spinal anaesthesia. The anaesthesia consultant feels general anaesthesia is safer to prevent any possible introduction of virus from blood or skin rashes directly to CSF.Can you advise on this controversy.
(answered 03/07/2003)

diptheroids in sputum
(answered 02/27/2003)

I am interested in working in bioterrorism preparedness. I am a medical technologist with 16 years of experience primarily in clinical microbiology. I also have a Master of Health Science degree. What resources are available for finding jobs in this field for someone with my level of experience, education and strong desire to help protect our country? Most jobs listed in ASM News are for doctoral level microbiologists and I was wondering how someone of a lower education level could contribute in this area.
(answered 02/18/2003)

What's the recommended rapid microbiology detection system that can also meet regulatory (e.g. FDA) requirements?
(answered 02/14/2003)

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