Parasitlogy

Parasitology

We want to switch from PVA to Protofix for ova and parasite collection. What validation is required? Do we need to collect samples from patients in duplicate? If so, how many positives/negatives? Is there another way to validate without involving patients?

(answered 05/15/2007)

Would you give us directions for a trichrome method of staining Protozoa that works well using PVA with zinc fixative? Since giving up the mercury fixative, we have trouble getting good nuclear staining.
(answered 03/19/2007)

What percentage of the population has cryptosporidium?
(answered 11/08/2006)

Instructions from our reference laboratory for blood collection for the filariasis antigen recommends that the blood be collected between 10 p.m. and 2 a.m. Why does this time frame increase the quality of the result?
(answered 11/05/2006)

Should you vortex or gentley shake prep for trichomonas set mount?
(answered 10/11/2006)

What is/are the recommended methods to screen for trichomonas vaginalis from genital swabs?
(answered 06/30/2006)

What is the best method for specimen processing fron SAF for Staining with Trichrome?
(answered 06/29/2006)

How common is Dientamoeba fragilis infection in children in the USA? Would a slide for R/O D.fragilis make sense on all ped pt having a giardia screen ?
(answered 03/03/2006)

ARE THERE ANY SCREENING TESTS FOR BABESIA SPECIES?
(answered 02/27/2006)

Often we are asked to speciate between Trichomonas hominis and Trichomas vaginalis . Other than doing molecular testing is there any way looking at the microscopic that the Microbiology lab can speciate?
(answered 02/24/2006)

Regarding the test Ova and Parasites, since both a concentration and a permanent smear (in our case Trichrome) is required by the College of American Pathologists, can we use both the CPT Code 87177 (concentration) AND 88313 (Trichrome) for each specimen? Thank you!
(answered 12/23/2005)

How do I locate a specialist in cerebral cysticercosis
(answered 12/09/2005)

HOW OFTEN DOES TRICHROME QC NEED TO BE RUN
(answered 09/15/2005)

Is ther a PCR test for Entamoeba histolytica? The specimen is bone.
(answered 08/10/2005)

Is there a PCR test for Entamoeba histolytica? Can it be used on bone?
(answered 08/10/2005)

why is methanol used as a fixative in blood staining? what is its proper mode of action?
(answered 08/10/2005)

Does PVA fixative render fecal material noninfectious? Our hazardous waste disposal company is requiring us to certify that the PVA vials used for parasitology are noninfectious and state the disinfectant (Schaudinn's fixative, perhaps?) before they will pick them up and dispose of them.
(answered 08/10/2005)

I am searching for a good guide to identify ticks and other vectors. What book(s) would you recommend?
(answered 06/28/2005)

Does anyone have experience with the Xpect kit for giardia/crypto testing?
(answered 05/16/2005)

WHAT IS THE RECOMMENDED STAIN FOR BLOOD PARASITES? IS THERE A BENEFIT TO DOING A WRIGHT-GEIMSA VS JUST GIEMSA.
(answered 04/26/2005)

Hello, I am a teacher of biology in a secondary school in Ghana and i would like to obtain information about how to test the effectiveness of anti malarial drugs being sold on the market. This is because the parasite is know to have become resistant to many of these drugs.I want a detailed scientific explanation. A student asked this question and would like you to help me out. Hoping to hearing from you. Thanks
(answered 03/02/2005)

why there is no antibody test for plasmodium sp.? (unlike babesia microti for instance who has an indirect IF test)
(answered 02/02/2005)

I have a question about a previous answer relating to CPT coding of parasite exams that was answered on 08/03/04. It was indicated to use 88312 as the new correct code for the trichrome. In reading the 2005 CPT coding book, it still specifically lists trichrome as 88313. Can you please clarify this? thanks
(answered 02/02/2005)

What is the time period after passage of stool does it need to be placed in preservative for both culture and Ova and Parasite testing?
(answered 12/20/2004)

I am looking for information on the detection of Giardia lamblia and Cryptosporidium parvm from sewage, not water, and cannot find it. I was wondering if you had any answers or could tell me where I can find some answers?
(answered 06/02/2004)

We recently found only Giardia trophs on an O&P exam, and the Merifluor Giardia antigen was negative. This made us question the protocol to "screen" for Giardia first before ordering a full O&P when trophs will be missed by this assay. Should we include a disclaimer of some sort on our reports?
(answered 05/29/2004)

Has anyone performed validation studies of the Genzyme "OSOM Trichomonas Rapid Test"?
(answered 05/05/2004)

I need a pinworm procedure. I am in the process of updating my Operational manual and I do not have this procedure.
(answered 04/27/2004)

from what tissues or fluids would you isolate the malaria parasite from ? How would You take this sample
(answered 04/06/2004)

We have recently become CAP accredited and are required to perform trichrome stains on all O&Ps. We had previously performed them only if the physician requested "look for amoebae" or if the technologist saw something suspicious on the direct or concentrated wet mount. We are being denied reimbursement for the CPT code 88313 which is specifically stated on page 277 of the 2003 CPT Code Book as the correct code to use. The lack of reimbursement was not a great concern when we performed the test infrequently, but due to the significantly increased workload, we would like to be able to recover the cost. Can you suggest an alternative code that is acceptable to Medicare or is there a way to state a policy acceptable to CAP that will keep us from having to do this test on all specimens? After adding this test and looking at more than 500 slides, we have yet to find one that was not indicated by what was seen on the wet mounts.
(answered 10/15/2003)

I am a student studying in London in tropical diseases and have heard about a type of nematode parasite that resmebles hairs,fibres,eyelashes and crystals depending on where it is on the human body and what metamorphic stage they are at. This is called unidentified skin parasite infection and there is only three pages in total on the internet on it. Can it be transferred by breathing ie airborne, does it kill the human host and if so how long, I heard that someone went deaf from it, is that possible? What are the best treatments of slowing down the effects. Did it really stem from being a parasite on a scabies or crab lice and if so how does it get so big? How can there be no treatment?
(answered 10/15/2003)

WHAT IS THE MOST CURRENT RULING ON THE USE OF PVA AND FORMALIN IN THE LAB AND SAFE DISPOSAL?
(answered 08/27/2002)

HOW DOES MAN ACQUIRE LEPTOSPIROSIS AND WHAT HAPPENS TO HIM ?
(answered 08/07/2003)

Our facility performs many O&P exams with very few positve results. We also have an immunoassay for Cryptosporidium and Giardia. I believe those are the most likely parasites to be found in the southeast and that we should be screening with the immunoassay instead of performing the ova and parasite examination. I would like to reserve the ova and parasite examination for when a specific parasite outside of Giardia or Crypto is suspected and/or when the patient has a relevant travel history. Any thoughts on the subject. Does anyone know of a particular article(s) that refer to this subject.
(answered 08/07/2003)

Do you know where to find parasitology teaching slides?
(answered 07/08/2003)

What is the stability (so it can be observed) of Trichomonas vaginalis in a vaginal discharge sample or in swab inmersed in saline, kept in a tube?
(answered 07/03/2003)

Pneumocystis Taxonomy Could you please confirm a rumour that the organism previously called Pneumocystis carinii is now called Pneumocystis jiroveci.
(answered 06/25/2003)

Do most labs perform cultures for Strongyloides?
(answered 06/17/2003)

What is the magnification of the pinworm eggs on your website at www.asmusa.org/division/c/photo/pinworm2.jpg and pinworm2.jpg?
(answered 05/14/2003)

Which triage kits can be used to detect E. Histolytica in a liver abscess specimen?
(answered 04/07/2003)

Our Parasitology lab is being asked to stop using 100% ethanol if at all possible. Is there a substitute for it when doing Trichrome stains?
(answered 03/21/2003)

WHAT IS THE BEST TRANSPORT MEDIA TO USE FOR STOOL SPECIMENS
(answered 02/18/2003)

back to Archive Index