Friday, 12 May 2017 15:19

Next-Generation Sequencing in the Clinical Microbiology Lab

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Last week’s Bugs & Drugs post presented the benefits of using molecular technologies in diagnosing infectious diseases. “Molecular technologies” is a catch-all phrase for many different methods to look at the genetic content of a microbial infection, and one of the methods that is quickly increasing in clinical applications is next-generation sequencing (NGS). NGS itself is a term for the collective sequencing techniques and their associated analytical pipelines that have been developed since 454 pyrosequencing advanced the decades-old Sanger sequencing protocols.

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As previously discussed on the blog, development of new clinical assays requires standardization and validation to ensure the technique providess accurate results in a variety of locations and conditions. As the technology has been developed and applied toward basic research, ASM has facilitated conversations about its transfer to clinical uses. In 2016, the American Academy of Microbiology released a Colloquium Report entitled “Applications of Clinical Microbial Next-Generation Sequencing.” This Report summarized the current state of NGS in the clinic and made recommendations for further implementation.

Now several of the experts involved in the Report, together with ASM and the College of American Pathologists, have published an open-access update to the report. The article addresses assay design and validation using concrete examples from two separate studies, one using respiratory specimens and one using cerebrospinal fluid specimens. In addition to wet bench protocols, the article outlines important aspects of database selection and curation—aspects that can strongly influence the analytical outcome of a sequencing assay.

AAM Colloquium Report: Applications of Clinical Microbial Next-Generation Sequencing 

Arch Pathol Lab Med: Validation of Metagenomic Next-Generation Sequencing Tests for Universal Pathogen Detection

Looking for more resources on NGS in the clinical lab? Try these reviews from the Journal of Clinical Microbiology:

And don’t miss ASM’s Molecular Diagnostics course, starting June 14th. Learn more about this event and how to register for this web-based learning opportunity here:

Molecular Diagnostics: Applications for the Diagnosis of Infectious Disease

ASM Microbe has many sessions on molecular diagnostic tools, including next-generation sequencing. Be sure to register today to participate in these cutting-edge sessions!

ASM Microbe

Last modified on Monday, 15 May 2017 10:31

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