The Gilmore lab investigates translocation of proteins across and integration of membrane proteins into the endoplasmic reticulum. They are also investigating asparagine-linked glycosylation of newly synthesized proteins by the oligosaccharyltransferase (OST). Their current research in the area of protein translocation and membrane protein integration utilizes the S. cerevisiae experimental system to investigate the gating mechanism of the protein translocation channel (Sec61 complex) by ribosome-nascent polypeptide complex that has been targeted to the ER membrane by the signal recognition particle. Current studies on the oligosaccharyltransferase utilize siRNA technology to achieve selective depletion of OST subunits in mammalian cells. These experiments are directed towards understanding how the STT3A and STT3B isoforms of the OST complex cooperate to mediate optimal N-linked glycosylation of newly synthesized proteins.