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Tuesday, 17 January 2017 13:47

Researchers Use Innovative Methods to Study Vertical Transmission of Microbes

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Nicola Segata and colleagues with the Computational Metagenomics laboratory at the University of Trento’s Centre for Integrative Biology (CIBIO) in Italy study several aspects of the microbiome.

“One of the questions we asked is, ‘Where does the infant microbiome come from in the first place?” Segata said. “While for some microorganisms this has been investigated using traditional methodologies for some time, with our background in computational metagenomics, we wondered if we could use noncultivation-based techniques to better address this question.” (Photos ©Alessio Coser for University of Trento)

It has been assumed that mothers pass on gut microbes to their infants during and just after delivery, a process called vertical transmission, but because of technical restraints, the evidence of this occurring has been limited. Previous cultivation-free studies have observed the same microbial species within mothers and infants and therefore assumed that transmission occurred “but in reality, unless you can see the exact same strain or genetic variant, it’s very hard to conclude that,” said study co-senior author Adrian Tett, PhD, a senior research associate.

In work published in mSystems this week, Segata, Tett and colleagues successfully combined laboratory and novel computational techniques to systematically track the vertical transmission of microbes in a pilot study. They found several identical bacterial strains in the microbiomes of both infants and their mothers that were distinct from those found in other infants or mothers, a sign of vertical transmission. In addition, transmitted strains from the Bacteroides and Bifidobacterium species were found to be active in both the mother and infant gut microbial communities.

mSystems Report: Studying Vertical Microbiome Transmission from Mothers to Infants by Strain-Level Metagenomic Profiling

“We developed methods to identify the vertical flow of microorganisms from mothers to infants and showed that mothers are sources of microbes that might be important in the development of the infant gut microbiome,” Segata said.

The investigators collected fecal and breast milk samples from five mother-infant pairs recruited by a local hospital when the infants were three months old. They collected additional samples from two of the mother-infant pairs again when the infants were 10 months old, and from another mother-infant pair when the baby was 16 months old. They applied shotgun metagenomic sequencing to 24 microbiome samples (eight mother fecal samples, eight infant fecal samples and eight breast milk samples) to see which microbes were present. They also used metatranscriptomics in fecal samples from two of the pairs to see which microbes were active.

Initially, as expected, the mothers’ intestines had greater microbial diversity than those of the infants. However, the gut microbiome of the 16-month-old child had shifted toward a more mother-like composition with an increase in microbial diversity. Breast milk samples had limited diversity shortly after birth; skin microbes were observed in only low numbers in the infants’ gut microbiomes, suggesting that skin microbes do not colonize the human gut.

To analyze microbial transmission from mother to infant, the team further analyzed the metagenomic samples at a finer level to assess specific bacterial strains using newly developed computational approaches. One infant harbored a strain of the common infant bacterium Bifidobacterium bifidum that was 99.96% identical to his mother’s but clearly distinct from B. bifidum strains seen in the other infants and mothers, offering strong evidence for vertical microbial transmission. Another infant had strains of two other bacteria, Coprococcus comes and Ruminococcus bromii, that were over 99% identical to his mother’s. Importantly, from metatranscriptomics, researchers observed some of the same strains of bacteria active in both the mothers’ and the infants’ guts.

While the results are still early, Segata said, “there is probably a substantial fraction of bacteria in the infant that come from the mothers.” The team is now studying more mother-infant pairs from the time of the babies’ births to over one year of age, including analyses of the mothers’ and infants’ microbiomes from several body locations. They will compare microbe transmission routes during vaginal and Cesarean section deliveries, breastfeeding, and skin-to-skin contact shortly after birth.

The study was funded by Fondazione CARITRO, a local foundation, and supported by the Italian Ministry of Education, Universities and Research and by the European Commission.

Last modified on Tuesday, 17 January 2017 16:31
Karen Blum

Karen Blum, a writer in the Baltimore area, has been covering health and science for 20+ years. Her work has been published in daily newspapers including The Baltimore Sun and The Palm Beach Post; national news websites like and; and magazines and news services for health professionals including Anesthesiology News, Pharmacy Practice News, Internal Medicine News, and

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